Reagentless amperometric glucose biosensor based on the immobilization of glucose oxidase on a ferrocene@NaY zeolite composite
Reagentless amperometric glucose biosensor based on the immobilization of glucose oxidase on a ferrocene@NaY zeolite composite
复制标题
基于二茂铁@NaY沸石复合材料上葡萄糖氧化酶固定化的无试剂电流型葡萄糖生物传感器
DOI：
10.1007/s00604-011-0624-1
复制
发表时间：
2011-09-01
影响因子：
5.7
通讯作者：
Sun, Youbao
中科院分区：
文献类型：
--
作者：
Dong, Junping;Zhou, Xiaojuan;Sun, Youbao
Ferrocene (Fc) was encapsulated in the cavities of a NaY zeolite by vapor diffusion via sublimation at below 100 A degrees C. The resulting Fc@NaY zeolite composite was investigated by power X-ray diffraction, diffuse reflectance UV-vis and FT-IR spectroscopy, and by cyclic voltammetry. The results indicated that Fc was encapsulated into the zeolite whose microporous structure had remained intact. The Fc in the silica matrix had retained its electroactivity and did not leach out. A glucose biosensor was obtained by immobilization of the modified zeolite and glucose oxidase on a carbon paste electrode. It displays a linear response to glucose (from 0.8 mu M to 4.0 mM), a detection limit of 0.2 mu M, and a response time of 4 s. The good performance of the biosensor is ascribed to the biocompatibility of the zeolite and presence of Fc which facilitates the electron transfer from the enzyme to the surface of the electrode.
