A class B scavenger receptor mediates antimicrobial peptide secretion and phagocytosis in Chinese mitten crab (Eriocheir sinensis)
A class B scavenger receptor mediates antimicrobial peptide secretion and phagocytosis in Chinese mitten crab (Eriocheir sinensis)
复制标题
B 类清道夫受体介导中华绒螯蟹（Eriocheir sinensis）抗菌肽的分泌和吞噬作用。
DOI：
10.1016/j.dci.2019.103496
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发表时间：
2020-02-01
影响因子：
2.9
通讯作者：
Li, Weiwei
中科院分区：
文献类型：
--
作者：
Tang, Muxue;Li, Xuejie;Li, Weiwei
清道夫受体（SR）是对先天免疫至关重要的模式识别受体（PRR）。除了它们在免疫识别、微生物吞噬和修饰的内源性分子的清除中的重要性之外，它们还作为共受体激活下游免疫应答。本研究在中华绒螯蟹（Eriocheir sinensis）中鉴定了一种B类清道夫受体（EsSR-B2）。该基因全长2,517 bp，编码517个氨基酸。EsSR-B2在所有测试的组织中广泛表达，并由微生物刺激诱导。重组EsSR-B2在体外与细菌和病原体相关分子模式结合在敲低EsSR-B2和用金黄色葡萄球菌或副溶血性弧菌进行细菌攻击后，血细胞中的吞噬率降低。此外，在EsSR-B2沉默后，响应于不同微生物刺激的几种抗微生物肽（AMP）的表达降低。因此，EsSR-B2是保护E.中华绒螯蟹通过促进吞噬作用和增强AMP表达来抵抗入侵病原体。
Scavenger receptors (SRs) are pattern recognition receptors (PRRs) vital for innate immunity. As well as their importance in immune recognition, microbe phagocytosis, and the clearance of modified endogenous molecules, they also activate downstream immune responses as co-receptors. In the current study, we identified a class B scavenger receptor in Eriocheir sinensis (EsSR-B2). The full-length gene is 2,517 bp and encodes a 517 amino acid polypeptide. EsSR-B2 is expressed widely in all tested tissues and is induced by microbial stimulation. Recombinant EsSR-B2 binds to bacteria and pathogen-associated molecular patterns in vitro. Upon knockdown of EsSR-B2 and bacterial challenge with Staphylococcus aureus or Vibrio parahaemolyticus, phagocytosis rates in hemocytes are decreased. Moreover, the expression of several antimicrobial peptides (AMPs) in response to distinct microorganism stimulation is decreased following EsSR-B2 silencing. Thus, EsSR-B2 is a PRR that protects E. sinensis against invading pathogens by promoting phagocytosis and enhancing AMP expression.