Iron-histidine stretching Raman lines of the aa3-type cytochrome oxidases
Iron-histidine stretching Raman lines of the aa3-type cytochrome oxidases
复制标题
aa3 型细胞色素氧化酶的铁-组氨酸拉伸拉曼线
DOI：
10.1021/ja00364a072
复制
发表时间：
1983
影响因子：
15
通讯作者：
T. Kitagawa
中科院分区：
文献类型：
--
作者：
T. Ogura;K. Hon;T. Oshima;S. Yoshikawa;T. Kitagawa
Figure 1. Resonance Raman spectra of 56Fe-(A) and 54Fe-substituted (B) reduced cytochrome oxidase of T. thermophilus HB8 and its reduced and cyanide-bound form (C) excited at441. 6 nm. Enzyme was dissolved in a 20 mM Tris/HCl buffer (pH 7.7) containing 0.5% Triton X-100 and 0.1 mM EDTA. Heme a concentration was ca. 20 µ. Reduction of the enzyme was performed by addition of a minimal amount of solid dithionite after mixing ascorbate (final concentration 50 mM) and N,-
