Contribution of net hepatic glycogenolysis to glucose production during the early postprandial period
Contribution of net hepatic glycogenolysis to glucose production during the early postprandial period
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DOI：
10.1152/ajpendo.1996.270.1.e186
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发表时间：
1996-01-01
影响因子：
5.1
通讯作者：
Shulman, GI
中科院分区：
文献类型：
--
作者：
Petersen, KF;Price, T;Shulman, GI
在13名健康志愿者中，通过使用C-13核磁共振光谱法无创测量肝糖原含量，研究了禁食前12小时内净肝糖原分解和再生对葡萄糖产生的相对贡献。通过将肝糖原含量的变化乘以磁共振成像测定的肝脏体积来计算净肝糖原分解率。通过输注[6，6-H-2]-葡萄糖测定的葡萄糖生成速率与净肝糖原分解速率之间的差异计算肝糖原生成速率。在下午6点给予液体混合餐（1，000 kcal; 60%葡萄糖），向其中加入[2-H-2]葡萄糖以跟踪葡萄糖吸收。在晚上11点至凌晨1点和凌晨3点至6点之间测量肝糖原含量。在晚上11点，浓度为470 mM，并在夜间线性下降。平均肝脏体积为1.47 +/- 0.06升。净肝糖原分解（5.8 +/- 0.8 μ mol . kg体重（-1）。min（-1））平均占全身葡萄糖产生速率（12.6 +/- 0.6 μ mol . kg体重（-1）。min（-1））。总之，这项研究表明，即使在吸收后阶段的早期，肝糖原储备最大，肝糖原生成的贡献类似于50%的肝葡萄糖生产。
Relative contributions of net hepatic glycogenolysis and gluconeogenesis to glucose production during the first 12 h of a fast were studied in 13 healthy volunteers by noninvasively measuring hepatic glycogen content using C-13 nuclear magnetic resonance spectroscopy. Rates of net hepatic glycogenolysis were calculated by multiplying the change in liver glycogen content with liver volume determined by magnetic resonance imaging. Rates of gluconeogenesis were calculated as the difference between rates of glucose production determined with an infusion of [6,6-H-2]-glucose and net hepatic glycogenolysis. At 6 P.M. a liquid mixed meal (1,000 kcal; 60% as glucose) was given, to which [2-H-2]glucose was added to trace glucose absorption. Hepatic glycogen content was measured between 11 P.M. and 1 A.M. and between 3 and 6 A.M. At 11 P.M. the concentration was 470 mM and it decreased linearly during the night. The mean liver volume was 1.47 +/- 0.06 liters. Net hepatic glycogenolysis (5.8 +/- 0.8 mu mol . kg body wt(-1) . min(-1)) accounted for, on average, 45 +/- 6% and gluconeogenesis for 55 +/- 6% of the rate of whole body glucose production (12.6 +/- 0.6 mu mol . kg body wt(-1) . min(-1)). In conclusion, this study shows that, even early in the phase of the postabsorptive period when liver glycogen stores are maximal, gluconeogenesis contributes similar to 50% to hepatic glucose production.