Skin-Nerve Co-culture Systems for Disease Modeling and Drug Discovery.
Skin-Nerve Co-culture Systems for Disease Modeling and Drug Discovery.
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DOI：
10.1089/ten.tec.2020.0296
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发表时间：
2020-12
期刊：
影响因子：
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通讯作者：
S. Schutte;Feni Kadakia;S. Davidson
中科院分区：
文献类型：
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作者：
S. Schutte;Feni Kadakia;S. Davidson
与皮肤神经界面相关的突出临床问题包括屏障功能障碍和红斑，但正是疼痛和瘙痒的症状导致患者就医。组织工程神经支配皮肤模型为研究神经皮肤疾病的机制、药物筛选和皮肤设备开发提供了极好的解决方案。神经支配的皮肤替代品为传统的单层培养提供了解决方案，并且具有使其在某些应用（例如测量体感）中更适合动物研究的优点。组织工程神经支配皮肤模型复制了复杂的分层表皮，为天然皮肤提供屏障功能，这是单层共培养物所缺乏的功能，同时允许测量动物模型中无法实现的神经功能。在这篇综述中，将讨论不同细胞来源和支架材料的优点和缺点，并回顾该领域的现状。
Prominent clinical problems related to the skin-nerve interface include barrier dysfunction and erythema but it is the symptoms of pain and itch that lead patients to seek medical treatment. Tissue-engineered innervated skin models provide an excellent solution for studying the mechanisms underlying neuro-cutaneous disorders for drug screening, and cutaneous device development. Innervated skin substitutes provide solutions to the traditional monolayer cultures and have advantages that make them preferable to animal studies for certain applications, such as measuring somatosensation. The tissue-engineered innervated skin models replicate the complex stratified epidermis that provides barrier function in native skin, a feature that is lacking in monolayer co-cultures, while allowing for measurement of nerve function that cannot be achieved in animal models. In this review, the advantages and disadvantages of different cell sources and scaffold materials will be discussed and a presentation of the current state of the field is reviewed.