Telemetric animal model to evaluate visceral pain in the freely moving rat
Telemetric animal model to evaluate visceral pain in the freely moving rat
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DOI：
10.1016/s0304-3959(03)00170-2
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发表时间：
2003-09-01
期刊：
影响因子：
7.4
通讯作者：
Meulemans, AL
中科院分区：
文献类型：
--
作者：
Nijsen, MJMA;Ongenae, NGH;Meulemans, AL
几个研究小组通过肌电图(EMG)测量了清醒束缚、包裹或轻度麻醉的大鼠对内脏扩张的内脏运动反应。我们的目标是开发一种更具生理性和无压力的技术，能够同时测量清醒、自由活动的大鼠十二指肠扩张引起的内脏运动和心血管反应。一种由双极电极对和动脉导管组成的遥测发射机被长期植入大鼠体内，以测量腹部肌电、平均动脉压(MAP)和心率(HR)。此外，在十二指肠内慢性放置球囊导管，以提供容量固定的阶梯(0.1-0.6ml)或相性(0.1、0.3、0.5ml)的扩张。分析扩张时的曲线下面积(AUC，MV S)和最大波幅(EMG(Max)，MV)。通过预先给予吗啡(0.3、1.5和3 mg/kg，腹腔注射)来验证模型的有效性。阶梯和阶段性扩张使AUC和EMG(Max)、HR和MAP呈体积依赖性增加。吗啡预先处理可抑制扩张引起的内脏运动反应，即腹部收缩，AUC和EMG(Max)的增加。这些发现表明，遥测是一种适当的工具，可以在大鼠笼子里连续和同时测量内脏运动和心血管对有害的十二指肠扩张的反应，而不需要额外的处理相关或束缚诱导的压力。所建立的动物内脏模型旨在研究新药物化合物的急性和慢性镇痛特性。(C)2003年国际疼痛研究协会。爱思唯尔出版，版权所有。
Several research groups have measured the visceromotor response to visceral distension by electromyography (EMG) in the conscious restraint, wrapped or lightly anaesthetized rat. Our aim was to develop a more physiological and stress-free technique that enables the simultaneous measurement of duodenal distension-induced visceromotor and cardiovascular responses in the conscious, freely moving rat. A telemetry transmitter, consisting of a bipolar electrode pair and arterial catheter, was chronically implanted into the rat to measure abdominal EMG, mean arterial pressure (MAP) and heart rate (HR). Furthermore, a balloon catheter was chronically implanted in the duodenum to deliver volume-fixed staircase (0.1-0.6 ml) or phasic (0.1, 0.3, 0.5 ml) distensions. The area under the curve (AUC., mV s) and maximal amplitude (EMG(max), mV) during distension were analyzed. The model was validated by pre-treatment with morphine (0.3, 1.5 and 3 mg/kg, intraperitoneally). Staircase and phasic distension produced a volume-dependent increase in AUC and EMG(max), HR and MAP. Pre-treatment with morphine inhibited the distension-induced visceromotor response, i.e. abdominal contractions, increase in AUC and EMG(max). These findings indicate that telemetry is an adequate tool to measure visceromotor and cardiovascular responses to averse, noxious duodenal distension continuously and simultaneously in the rats home cage, without additional handling-related or restraint-induced stress. The presented animal visceral model is intended for studying acute and chronic analgesic properties of new pharmaceutical compounds. (C) 2003 International Association for the Study of Pain. Published by Elsevier B.V. All rights reserved.