Integration of genome wide association studies and co-expression networks reveal roles of PtoWRKY 42-PtoUGT76C1-1 in trans-zeatin metabolism and cytokinin sensitivity in poplar
Integration of genome wide association studies and co-expression networks reveal roles of PtoWRKY 42-PtoUGT76C1-1 in trans-zeatin metabolism and cytokinin sensitivity in poplar
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全基因组关联研究和共表达网络的整合揭示了 PtoWRKY 42-PtoUGT76C1-1 在杨树反式玉米素代谢和细胞分裂素敏感性中的作用
DOI：
10.1111/nph.17469
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发表时间：
2021
期刊：
影响因子：
9.4
通讯作者：
Zhang Deqiang
中科院分区：
文献类型：
--
作者：
Song Yuepeng;Chen Panfei;Xuan Anran;Bu Chenhao;Liu Peng;Ingvarsson Par K.;El-Kassaby Yousry A.;Zhang Deqiang
细胞分裂素在植物离体再生中起重要作用。细胞分裂素N-葡萄糖苷通过不可逆糖基化途径产生，该途径调节内源性细胞分裂素含量。虽然细胞分裂素N-葡萄糖苷途径已经在高等植物中被发现，但迄今为止还没有鉴定出调节剂。我们进行了代谢组全基因组关联研究（mGWAS），加权基因共表达网络分析（WGCNA），和表达数量性状核苷酸（eQTN）作图，以构建核心三重遗传网络反式玉米素N-葡萄糖苷（ZNG）的（mGWAS基因表达表型）结果表明，毛白杨ZNG含量的变化主要是由于ZNG的代谢产物的差异，PtoWRKY 42是WRKY多基因家族IIb组成员。功能分析表明，PtoWRKY 42通过直接结合UDP-糖基转移酶76 C 1 - 1（PtoUGT 761 - 1）启动子的W-盒负调控ZNG积累。此外，PtoWRKY 42强烈诱导叶片衰老，6-BA，创伤，盐胁迫，导致ZNG水平降低。我们鉴定了PtoWRKY 42，细胞分裂素N-葡萄糖苷的负调控因子，其有助于ZNG水平的自然变化，并通过直接调节关键糖基转移酶基因PtoUGT 76 C1 - 1介导ZNG积累。
Cytokinins are important forin vitroshoot regeneration in plants. Cytokinin N‐glucosides are produced via an irreversible glycosylation pathway, which regulates the endogenous cytokinin content. Although cytokinin N‐glucoside pathways have been uncovered in higher plants, no regulator has been identified to date.We performed a metabolome genome‐wide association study (mGWAS), weighted gene co‐expression network analysis (WGCNA), and expression quantitative trait nucleotide (eQTN) mappings to build a core triple genetic network (mGWAS–gene expression–phenotype) for thetrans‐zeatin N‐glucoside (ZNG) metabolite using data from 435 unrelatedPopulus tomentosaindividuals.Variation of the ZNG level in poplar is attributed to the differential transcription ofPtoWRKY42, a member of WRKY multigene family group IIb. Functional analysis revealed thatPtoWRKY42negatively regulated ZNG accumulation by binding directly to the W‐box of the UDP‐glycosyltransferase 76C 1‐1 (PtoUGT761‐1) promoter. Also,PtoWRKY42was strongly induced by leaf senescence, 6‐BA, wounding, and salt stress, resulting in a reduced ZNG level.We identifiedPtoWRKY42, a negative regulator of cytokinin N‐glucosides, which contributes to the natural variation in ZNG level and mediates ZNG accumulation by directly modulating the key glycosyltransferase genePtoUGT76C1‐1.