Efficacy of Tetrandrine on Lowering Intraocular Pressure in Animal Model With Ocular Hypertension
Efficacy of Tetrandrine on Lowering Intraocular Pressure in Animal Model With Ocular Hypertension
复制标题
粉防己甲素对高眼压动物模型的降眼压作用
DOI：
10.1097/ijg.0b013e3181d7882a
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发表时间：
2011-03-01
影响因子：
2
通讯作者：
Zhang, Chun
中科院分区：
文献类型：
--
作者：
Huang, Ping;Xu, Yongsheng;Zhang, Chun
目的比较外用粉防己碱（从中药防己碱中分离得到的生物碱）与0.5%噻吗洛尔滴眼液对正常眼压和眼压高血压大鼠降低眼内压（IOP）的作用。方法实验设计为2部分。第一部分，将正常雄性Sprague-Dawley大鼠分为4组，右眼局部注射0.1%、0.2%、0.3%粉防己碱和0.5%噻吗洛尔各1次，对侧眼局部注射0.9%生理盐水1次作为对照。第二部分，通过二极管激光治疗单侧诱导大鼠高眼压症模型。将上述药物每日两次施用于高血压眼。对照组给予0.9%生理盐水。 TonoPen XL 眼压计用于测定 IOP 水平。结果粉防己碱治疗的血压正常的大鼠没有检测到对眼压的降低作用，而噻吗洛尔则显着降低血压正常的眼睛的眼压。与生理盐水治疗组相比，粉防己碱和噻吗洛尔均显着降低了高血压眼的眼压水平。本研究中使用的所有浓度的粉防己碱均显示出激光诱发的高血压眼的眼压显着降低。 0.3% 粉防己碱在降低高眼压眼中的眼压升高方面与 0.5% 噻吗洛尔具有相似的功效。结论本研究提供的证据表明粉防己碱对降低高眼压大鼠模型的眼压水平具有重要作用。粉防己碱的作用机制需要进一步研究。
PurposeTo compare the efficacy of topical tetrandrine (an alkaloid isolated from the Chinese medicinal herb Radix Stephania tetrandrae S) and timolol 0.5% ophthalmic solution on lowering the intraocular pressure (IOP) in ocular normotensive and hypertensive rats. MethodsThe experiment was designed as 2 parts. In the first part, normal male Sprague–Dawley rats were divided into 4 groups followed by topical administration once of 0.1%, 0.2%, 0.3% tetrandrine, and 0.5% timolol in the right eye, 0.9% saline was administered once at the opposite eye as control. In the second part, the ocular hypertension model was induced unilaterally in the rats by a diode laser treatment. Twice daily applications of the above drugs were delivered to hypertensive eyes. The control group was administered 0.9% saline. The TonoPen XL tonometer was used to determine the IOP levels. ResultsNo lowering effect on IOP was detected in the normotensive rats treated with tetrandrine whereas timolol significantly reduced IOP in normotensive eyes. Both tetrandrine and timolol significantly reduced the IOP levels in the hypertensive eyes compared with the levels in the saline-treated groups. All concentrations of tetrandrine used in this study showed significant reduction of IOP in the laser-induced hypertensive eyes. Tetrandrine 0.3% had a similar efficacy as 0.5% timolol in reducing elevated IOP in ocular hypertensive eyes. ConclusionsThis study provides evidence that tetrandrine has a major effect on lowering IOP levels in the ocular hypertension rat model. The functional mechanisms of tetrandrine require further investigation.