Spatiotemporal functional organization of excitatory synaptic inputs onto macaque V1 neurons
Spatiotemporal functional organization of excitatory synaptic inputs onto macaque V1 neurons
复制标题
猕猴 V1 神经元兴奋性突触输入的时空功能组织
DOI：
10.1038/s41467-020-14501-y
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发表时间：
2020-02-04
影响因子：
16.6
通讯作者：
Tang, Shiming
中科院分区：
文献类型：
--
作者：
Ju, Niansheng;Li, Yang;Tang, Shiming
突触输入到树突上的整合为神经元计算提供了基础。尽管最近的研究已经开始概述单个神经元突触输入的空间组织，但与特定神经功能相关的潜在原理尚未得到很好的理解。本研究利用遗传编码谷氨酸传感器对清醒猴子进行双光子树突成像，并以高时空分辨率绘制出V1浅层神经元树突上的兴奋性突触输入。我们发现在单个V1神经元的基底树突中定向选择和颜色选择输入之间存在功能整合和权衡。树突上的突触输入根据刺激特征在空间上聚集，但在多维特征空间中功能分散，这为树突分支上的局部特征整合提供了潜在的基础。此外，与基底树突输入相比，顶树突的接收野更大，响应潜伏期更长，表明顶树突在视觉信息加工的反馈整合中起主导作用。突触输入到树突上的整合为神经元计算提供了基础。在这里，作者使用基因编码的谷氨酸传感器对清醒的猴子进行双光子树突成像，并以高空间和时间分辨率绘制单个V1浅层神经元树突上的兴奋性突触输入。
The integration of synaptic inputs onto dendrites provides the basis for neuronal computation. Whereas recent studies have begun to outline the spatial organization of synaptic inputs on individual neurons, the underlying principles related to the specific neural functions are not well understood. Here we perform two-photon dendritic imaging with a genetically-encoded glutamate sensor in awake monkeys, and map the excitatory synaptic inputs on dendrites of individual V1 superficial layer neurons with high spatial and temporal resolution. We find a functional integration and trade-off between orientation-selective and color-selective inputs in basal dendrites of individual V1 neurons. Synaptic inputs on dendrites are spatially clustered by stimulus feature, but functionally scattered in multidimensional feature space, providing a potential substrate of local feature integration on dendritic branches. Furthermore, apical dendrite inputs have larger receptive fields and longer response latencies than basal dendrite inputs, suggesting a dominant role for apical dendrites in integrating feedback in visual information processing. The integration of synaptic inputs onto dendrites provides the basis for neuronal computation. Here the authors perform two-photon dendritic imaging with a genetically-encoded glutamate sensor in awake monkeys, and map the excitatory synaptic inputs on dendrites of individual V1 superficial layer neurons with high spatial and temporal resolution.