Trans-activation of HLA-DR gene by hepatitis B virus X gene product.
Trans-activation of HLA-DR gene by hepatitis B virus X gene product.
复制标题
乙型肝炎病毒X基因产物对HLA-DR基因的反式激活。
DOI：
10.1073/pnas.87.18.7140
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发表时间：
1990
影响因子：
11.1
通讯作者：
Siddiqui,A
中科院分区：
文献类型：
--
作者：
Hu,KQ;Vierling,JM;Siddiqui,A
B型肝炎病毒（HBV）感染期间的肝细胞损伤被认为是由人类白细胞抗原（HLA）限制性T淋巴细胞宿主对HBV抗原的免疫应答引起的。虽然HLA表达在肝脏炎症的存在下增强，但HBV本身是否能诱导受感染肝细胞上的HLA表达尚不清楚。在这项研究中，我们证明了诱导HLA-DR表达的人肝癌细胞系转染HBV DNA序列。单独HBV X基因可诱导HLA-DR表达。这种诱导与HLA-DR RNA升高相关，这是由HBV X蛋白对HLA-DR基因的转录反式激活直接导致的。这些研究表明，HBV X蛋白可以调节HLA-DR的表达，从而提高了X基因参与HBV感染的免疫发病机制的可能性。
Hepatocellular injury during hepatitis B virus (HBV) infection has been postulated to result from a human leukocyte antigen (HLA)-restricted T-lymphocyte host immune response against HBV antigens. Although HLA expression is enhanced in the presence of hepatic inflammation, whether HBV itself can induce HLA expression on infected hepatocytes is unknown. In this study, we demonstrate the induction of HLA-DR expression on human hepatoma cell lines transfected with HBV DNA sequences. The HBV X gene alone was capable of inducing HLA-DR expression. This induction correlated with elevated HLA-DR RNA, and this resulted directly from transcriptional trans-activation of the HLA-DR gene by the HBV X protein. These studies suggest that the HBV X protein can regulate the expression of HLA-DR and thus raise the possibility of participation by the X gene in the immunopathogenesis of HBV infection.