Comparative miRNAs analysis of Two contrasting broccoli inbred lines with divergent head-forming capacity under temperature stress.
Comparative miRNAs analysis of Two contrasting broccoli inbred lines with divergent head-forming capacity under temperature stress.
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DOI：
10.1186/s12864-015-2201-1
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发表时间：
2015-12-01
期刊：
影响因子：
4.4
通讯作者：
Huang HJ
中科院分区：
文献类型：
--
作者：
Chen CC;Fu SF;Norikazu M;Yang YW;Liu YJ;Ikeo K;Gojobori T;Huang HJ
MicroRNAs(MiRNAs)在转录后水平上对生长、发育和应激反应起着至关重要的作用。青花菜是一种重要的蔬菜作物，高温胁迫会降低青花菜的产量和品质。能在夏季高温下结球的西兰花自交系是台湾特有的品种。然而，对热胁迫下西兰花结球形成过程中miRNAome的了解有限。本研究对两个具有不同成头能力的近等基因系进行了分子特征研究。耐热青花菜在热胁迫下的结球能力强于热敏青花菜。通过深度测序和计算分析，20个已知的miRNAs在HT型和HS型之间显示了显著的差异表达。根据新miRNAs的注释标准，共鉴定出24个差异表达的新miRNAs序列。为了深入了解功能意义，预测了这44个差异表达的miRNAs中的213个独特的潜在靶点。这些靶标与顶端发育、阶段变化、对温度刺激的反应、激素和能量代谢有关。与HS相比，唯一的HT系的成头能力与Bo-FT基因的自主调控和热休克蛋白基因的表达水平较低有关。在基因比较中，一组miRNAs及其靶标在不同的HT型中具有一致的表达模式。这种对西兰花miRNAs及其潜在靶标的大规模表征是为了揭示miRNAs在耐热头部形成能力中的调节作用。本文的在线版本(doi：10.1186/s12864-0152201-1)包含补充材料，授权用户可以使用。
MicroRNAs (miRNAs) play a vital role in growth, development, and stress response at the post-transcriptional level. Broccoli (Brassica oleracea L. var italic) is an important vegetable crop, and the yield and quality of broccoli are decreased by heat stress. The broccoli inbred lines that are capable of producing head at high temperature in summer are unique varieties in Taiwan. However, knowledge of miRNAomes during the broccoli head formation under heat stress is limited. In this study, molecular characterization of two nearly isogenic lines with contrasting head-forming capacity was investigated. Head-forming capacity was better for heat-tolerant (HT) than heat-sensitive (HS) broccoli under heat stress. By deep sequencing and computational analysis, 20 known miRNAs showed significant differential expression between HT and HS genotypes. According to the criteria for annotation of new miRNAs, 24 novel miRNA sequences with differential expression between the two genotypes were identified. To gain insight into functional significance, 213 unique potential targets of these 44 differentially expressed miRNAs were predicted. These targets were implicated in shoot apical development, phase change, response to temperature stimulus, hormone and energy metabolism. The head-forming capacity of the unique HT line was related to autonomous regulation of Bo-FT genes and less expression level of heat shock protein genes as compared to HS. For the genotypic comparison, a set of miRNAs and their targets had consistent expression patterns in various HT genotypes. This large-scale characterization of broccoli miRNAs and their potential targets is to unravel the regulatory roles of miRNAs underlying heat-tolerant head-forming capacity. The online version of this article (doi:10.1186/s12864-015-2201-1) contains supplementary material, which is available to authorized users.