Erythrocyte membrane sidedness in lectin control of the Ca2+–A23187-mediated diskocyte \[rlarr2 ] echinocyte conversion
Erythrocyte membrane sidedness in lectin control of the Ca2+–A23187-mediated diskocyte \[rlarr2 ] echinocyte conversion
复制标题
凝集素控制 Ca2+–A23187 介导的盘细胞[rlarr2] 棘红细胞转化中的红细胞膜侧面性
作者：
R. Anderson;R. Lovrien
带离子载体A23187的人红细胞细胞质钙浓度升高，可促进细胞形态由盘状细胞向棘细胞转变1。这种转化与细胞内ATP水平密切相关，当钙通过细胞膜引入时，细胞内ATP水平下降2,3。在恢复正常ATP和钙水平后，棘刺细胞形态恢复为盘状细胞4,5。ATP对细胞形态的控制被认为依赖于一种蛋白激酶和磷酸酶的作用，这两种蛋白激酶和磷酸酶可以可逆地修饰细胞膜细胞骨架6 - 9。细胞骨架依次通过跨膜蛋白10 - 11与外膜表面接触。我们在这里发现，凝集素，小麦胚芽凝集素（WGA）与红细胞膜表面的结合阻断了diskocyte \[rlarr2]棘细胞在两个方向上的形态转化。WGA似乎是通过一种涉及跨膜糖蛋白糖蛋白13,14的机制起作用的。
Increasing the cytoplasmic calcium concentration of human erythrocytes with ionophore A23187 drives the transformation from diskocyte to echinocyte morphology1. This transformation is closely linked to the intracellular ATP level, which drops when calcium is introduced across the membrane2,3. The echinocyte morphology reverts to the diskocyte after restoration of normal ATP and calcium levels4,5. ATP control of cell morphology is thought to depend on the action of a protein kinase and phosphatase which reversibly modify the membrane cytoskeleton6–9. The cytoskeleton in turn is in contact with the outside membrane surface via transmembrane proteins10–11. We show here that binding of the lectin, wheat-germ agglutinin (WGA), to the erythrocyte membrane surface blocks the morphological conversions diskocyte \[rlarr2 ] echinocyte in both directions. WGA seems to work via a mechanism involving the transmembrane glycoprotein, glycophorin13,14.