Confirmation of a unique species of Giardia, parasitic in the quenda (Isoodon obesulus).
Confirmation of a unique species of Giardia, parasitic in the quenda (Isoodon obesulus).
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DOI：
10.1016/j.ijppaw.2016.01.002
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发表时间：
2016-04
期刊：
影响因子：
--
通讯作者：
Thompson RCA
中科院分区：
文献类型：
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作者：
Hillman A;Ash A;Elliot A;Lymbery A;Perez C;Thompson RCA
贾第虫的"quenda基因型"首先在quenda（syn.南部棕色袋狸，Isodon obesulus）。我们的目的是正式描述这种基因型作为一种贾第鞭毛虫，贾第鞭毛虫peramelis。75份粪便标本G. peramelis得自西澳大利亚州珀斯统计局的quenda。这些样品用于G. peramelis。PCR扩增和测序在18S rRNA和ITS1 - 5. 8s-ITS2位点最成功。系统发育分析将G. peramelis外部的'贾第鞭毛虫种复合体'和Giardia microti。这证实了G. peramelis，重新分类为贾第鞭毛虫的一个独立种。研究结果表明昆达线虫是G. peramelis。贾第鞭毛虫被正式描述。PCR技术对G. peramelis概述。G.提出了梭子蟹系统发育树。
The ‘quenda genotype’ of Giardia was first identified in quenda (syn. southern brown bandicoots, Isoodon obesulus) in Western Australia in 2004. We aimed to formally describe this genotype as a species of Giardia, Giardia peramelis. Seventy five faecal samples positive for G. peramelis were obtained from quenda within the Statistical Division of Perth, Western Australia. These samples were used in morphological and molecular characterisation of G. peramelis. PCR amplification and sequencing was most successful at the 18S rRNA and ITS1-5.8s-ITS2 loci. Phylogenetic analyses placed G. peramelis external to the ‘Giardia duodenalis species complex’ and Giardia microti. This confirmed the uniqueness of G. peramelis, warranting classification as a separate species of Giardia. Study findings suggest quenda are a natural host for G. peramelis. Giardia peramelis is formally described. PCR techniques for G. peramelis are outlined. G. peramelis phylogenetic trees are presented.