Unanticipated changes in steady-state mRNA levels for glyceraldehyde-3-phosphate dehydrogenase in rat tibiae.
Unanticipated changes in steady-state mRNA levels for glyceraldehyde-3-phosphate dehydrogenase in rat tibiae.
复制标题
大鼠胫骨中甘油醛-3-磷酸脱氢酶稳态 mRNA 水平的意外变化。
DOI：
10.1007/s00223-002-1098-2
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Turner,RT
中科院分区：
文献类型：
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作者：
Maran,A;Hefferan,TE;Zhang,M;Turner,RT
甘油醛-3-磷酸脱氢酶（GAPDH） mRNA水平通常被用作规范基因表达数据的内部控制，因为人们认为该基因是组成性表达的。然而，与L32 mRNA水平相比，后肢未负重的雌性大鼠胫骨中GAPDH mRNA水平增加了2.5倍以上。同样，与18S核糖体RNA相比，GAPDH mRNA水平升高。生长激素和酒精处理在GAPDH mRNA水平上没有差异，而在一些实验中，甲状旁腺激素和17β-雌二醇增加了GAPDH mRNA水平。综上所述，这些发现表明，在使用GAPDH基因作为内部控制之前，必须证明GAPDH的表达不会改变。
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA levels are commonly used as an internal control to normalize gene expression data based on the belief that this gene is constitutively expressed. However, GAPDH mRNA levels increased by more than 2.5-fold in tibiae of hindlimb unloaded female rats compared to L32 mRNA levels. Similarly, GAPDH mRNA levels increased compared to 18S ribosomal RNA. Treatment with growth hormone and alcohol show no disparity in GAPDH mRNA levels whereas in some experiments, parathyroid hormone and 17β-estradiol increased GAPDH mRNA levels. Taken together, these findings indicate that it is essential to demonstrate that GAPDH expression is not altered prior to using the gene as an internal control.