Activation, pronuclear formation, and development in vitro of pig oocytes following intracytoplasmic injection of freeze-dried spermatozoa
Activation, pronuclear formation, and development in vitro of pig oocytes following intracytoplasmic injection of freeze-dried spermatozoa
复制标题
DOI：
10.1095/biolreprod.104.031260
复制
发表时间：
2004-11-01
影响因子：
3.6
通讯作者：
Niwa, K
中科院分区：
文献类型：
--
作者：
Kwon, IK;Park, KE;Niwa, K
本文研究了猪卵母细胞经冷冻干燥精子胞浆内注射后的受精效果。激活和男性原核（MPN）的形成是更好的卵母细胞注射分离的冷冻干燥精子头比整个冷冻干燥精子，但分裂的胚胎一般难以发展到桑椹胚或囊胚期。精子冷冻干燥24 h后，卵母细胞的激活和精子头部注射后激活的卵母细胞中MPN的形成受到抑制。将冷冻干燥4 h并在4 ℃下保存的精子分离出的精子头注射后，胚胎才能发育到囊胚期。用Ca离子载体（5-10 μ M）处理注射的卵母细胞并没有增加发育到胚泡阶段的胚胎的比例。增加精子储存时间不影响卵母细胞激活或MPN形成，但仅在储存1个月后观察到囊胚发育。这些结果表明，猪卵母细胞可以与适当冷冻干燥的精子受精，受精卵可以发育到囊胚阶段。
The fertilization of pig oocytes following intracytoplasmic injection of freeze-dried spermatozoa was evaluated. Activation and male pronuclear (MPN) formation were better in oocytes injected with isolated freeze-dried sperm heads than whole freeze-dried spermatozoa, but cleaved embryos were generally difficult to develop to the morula or blastocyst stage. When spermatozoa were freeze-dried for 24 h, oocyte activation and MPN formation in activated oocytes after sperm head injection were inhibited. Embryo development to the blastocyst stage was only obtained after injecting sperm heads isolated from spermatozoa freeze-dried for 4 h and stored at 4degreesC. The proportion of embryos that developed to the blastocyst stage was not increased by the treatment of injected oocytes with Ca ionophore (5-10 muM). Increasing the sperm storage time did not affect oocyte activation or MPN formation, but blastocyst development was observed only after 1 mo of storage. These results demonstrate that pig oocytes can be fertilized with appropriately freeze-dried spermatozoa and that the fertilized oocytes can develop to the blastocyst stage.