Spatiotemporal distribution of antimicrobial resistant organisms in different water environments in urban and rural settings of Bangladesh
Spatiotemporal distribution of antimicrobial resistant organisms in different water environments in urban and rural settings of Bangladesh
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DOI：
10.1016/j.scitotenv.2022.154890
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发表时间：
2022-04-08
影响因子：
9.8
通讯作者：
Islam, Mohammad Aminul
中科院分区：
文献类型：
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作者：
Asaduzzaman, Muhammad;Rousham, Emily;Islam, Mohammad Aminul
临床重要的抗生素耐药细菌（ARB）及其相关基因的空间分布对于确定污染的环境分布和抗生素耐药（AMR）的“热点”具有重要意义。我们在孟加拉国农村家庭、农村家禽养殖场和城市食品市场三种环境中对饮用水、废水和地表水（河流和池塘）中的抗菌素耐药性进行了综合调查。利用ArcGIS软件，通过地理信息系统（GIS）进行空间制图。对397份样品进行了广谱产β -内酰胺酶大肠杆菌（ESBL-Ec）、耐碳青霉烯大肠杆菌（CR-Ec）和耐药基因（blaCTX-M-1、blaNDM-1）的检测。在农村家庭中，5%的饮用水供应样本检测出ESBLEc阳性，废水、池塘和河流水样中ESBL-Ec阳性的比例很高（分别为90%、76%和85%）。在家禽养殖场，10%的饮用水样本检测出ESBL-Ec阳性，而废水、池塘和河水的感染率很高（分别为90%、68%和85%）。与河水（33%）相比，生活污水和池塘水中的CR-Ec患病率相对较低（分别为8%和5%）。在城市地区，38%的饮用水样本和98%的食品市场废水样本检测出ESBLEc阳性，30%的废水样本检测出CR-Ec阳性。废水中ESBL浓度最高
The spatial distribution of clinically important antibiotic resistant bacteria (ARB) and associated genes is important to identify the environmental distribution of contamination and 'hotspots' of antimicrobial resistance (AMR). We conducted an integrated survey of AMR in drinking water, wastewater and surface water (rivers and ponds) in three settings in Bangladesh: rural households, rural poultry farms, and urban food markets. Spatial mapping was conducted via geographic information system (GIS) using ArcGIS software. Samples (n = 397) were analyzed for the presence of extended-spectrum beta-lactamase-producing Escherichia coli (ESBL-Ec), carbapenem-resistant E. coli (CR-Ec) and resistance genes (blaCTX-M-1, blaNDM-1). In rural households, 5% of drinking water supply samples tested positive for ESBLEc, and a high proportion of wastewater, pond and river water samples were positive for ESBL-Ec (90%, 76%, and 85%, respectively). In poultry farms, 10% of drinking water samples tested positive for ESBL-Ec compared to a high prevalence in wastewater, pond and river water (90%, 68%, and 85%, respectively). CR-Ec prevalence in household wastewater and pond water was relatively low (8% and 5%, respectively) compared to river water (33%). In urban areas, 38% of drinking water samples and 98% of wastewater samples from food markets tested positive for ESBLEc while 30% of wastewater samples tested positive for CR-Ec. Wastewaters had the highest concentrations of ESBL