High-resolution length sorting and purification of DNA-wrapped carbon nanotubes by size-exclusion chromatography
High-resolution length sorting and purification of DNA-wrapped carbon nanotubes by size-exclusion chromatography
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DOI：
10.1021/ac0508954
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发表时间：
2005-10-01
影响因子：
7.4
通讯作者：
Zheng, M
中科院分区：
文献类型：
--
作者：
Huang, XY;McLean, RS;Zheng, M
我们报告了一个尺寸排阻色谱法（SEC）的过程来纯化DNA包裹的碳纳米管（DNA-CNT），并将它们分成均匀长度的馏分。一种类型的硅基柱树脂被确定为显示最小的DNA-CNT吸附。三个这样的列串联的孔径为2000，1000，和300埃被发现分离DNA-CNT成非常窄的长度分布的馏分，直接通过原子力显微镜测量。随着长度的变化，平均长度从早期片段的> 500 nm单调减少到后期片段的< 100 nm
We report a size-exclusion chromatography (SEC) process to purify DNA-wrapped carbon nanotubes (DNA-CNT) and to sort them into fractions of uniform length. A type of silica-based column resin was identified that shows minimum adsorption of DNA-CNT. Three such columns in series with pore sizes of 2000, 1000, and 300 angstrom were found to separate DNA-CNT into fractions of very narrow length distribution, as measured directly by atomic force microscopy. The average length decreases monotonically from > 500 run in the early fractions to < 100 nm in the late fractions, with length variation