In vitro and in vivo antimicrobial efficacy of essential oils and individual compounds against Phytophthora parasitica var. nicotianae
In vitro and in vivo antimicrobial efficacy of essential oils and individual compounds against Phytophthora parasitica var. nicotianae
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DOI：
10.1111/jam.12208
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发表时间：
2013-07-01
影响因子：
4
通讯作者：
Yao, L.
中科院分区：
文献类型：
--
作者：
Lu, M.;Han, Z.;Yao, L.
目的评价紫苏、罗勒、天竺葵、香茅、孜然、百里香等植物精油及其主要成分对番茄疫霉菌的抑菌效果。烟草;研究肉桂醛处理后菌丝和孢子囊的形态变化，并进一步评价温室条件下对烟草黑胫病的潜在生物防治能力。方法与结果研究结果表明，EO对菌丝生长的抑制程度主要取决于EO的组成和浓度以及单个化合物的结构。肉桂醛对寄生疫霉菌丝生长、孢子囊形成、游动孢子的产生和萌发有明显的抑制作用。烟草在体外，实现完全抑制这些表型分别在72，36，36和18毫克L-1。扫描电镜观察发现，肉桂醛可引起菌丝和孢子囊的形态退化，如胞质凝聚、菌丝和孢子囊皱缩、菌丝和孢子囊壁肿胀和溶解等。肉桂醛的体内试验表明，该化合物在温室条件下对易感烟草植物的烟草黑胫病具有保护作用。结论离体和体内生物测定结果以及电镜观察结果与寄生青霉变种的显微结构一致。烟草碱支持在温室中使用肉桂醛作为有效的天然生物杀真菌剂的可能性。本研究为肉桂醛作为商业化杀菌剂或先导化合物开发利用提供了理论基础。烟草感染
Aim To evaluate the antimicrobial effects of essential oils (EOs) from cassia, basil, geranium, lemongrass, cumin and thyme, as well as their major components, against Phytophthora parasitica var. nicotianae; to investigate morphological changes in hyphae and sporangia in response to treatment with cinnamaldehyde; and to further evaluate potential biocontrol capacities against tobacco black shank under greenhouse conditions. Methods and Results The results revealed that the extent of mycelial growth inhibition was primarily dependent on the composition and concentration of the EOs and the structure of individual compounds. Cinnamaldehyde had a significantly higher inhibitory effect on mycelial growth, formation of sporangia, and production and germination of zoospores in P.parasitica var. nicotianae in vitro, achieving complete inhibition of these phenotypes at 72, 36, 36 and 18mg l-1, respectively. Scanning electron microscopic observations revealed that cinnamaldehyde can cause considerable morphological degenerations of hyphae and sporangia such as cytoplasmic coagulation, shrivelled mycelia and sporangia aggregates and swelling and lysis of mycelia and sporangia walls. In vivo assays with cinnamaldehyde demonstrated that this compound afforded protective effect against tobacco black shank under greenhouse conditions in susceptible tobacco plants. Conclusions The results of in vitro and in vivo bioassays, together with SEM imaging of the microstructure of P.parasitica var. nicotianae supported the possibility of using cinnamaldehyde as a potent natural biofungicide in the greenhouse. Significance and Impact of the Study This study provides a theoretical basis for the potential use of cinnamaldehyde as commercial agents or lead compounds that can be exploited as commercial biofungicides in the protection of tobacco plants from P.parasitica var. nicotianae infection.