Manual immunoephelometric assay of proteins, with use of polymer enhancement.
Manual immunoephelometric assay of proteins, with use of polymer enhancement.
复制标题
使用聚合物增强技术对蛋白质进行手动免疫光度分析。
作者：
J. Lizana;K. Hellsing
用普通荧光计作浊度计，建立了一种蛋白质的手动免疫比浊法。通过应用聚乙二醇（平均分子量，6000）对免疫反应的增强作用，在10分钟反应时间后定量白蛋白和纤维蛋白原。在目前条件下，灵敏度提高，从而减少抗血清消耗。反应条件进行了仔细研究，相对于最佳的时间和反应物的浓度。方法的精密度为2.6-3.6%（CV）。在白蛋白-抗白蛋白系统中的检测下限为0.1毫克/升。比较研究表明，尿白蛋白的自动免疫荧光测定法的相关系数为0.991，血浆纤维蛋白原的凝血酶凝块法的相关系数为0.908。我们特别建议使用这种手动免疫荧光法，其中小系列的样品进行分析。
A manual immunonephelometric method for proteins has been developed by using an ordinary fluorometer as a nephelometer. By applying the enhancing effect of polyethylene glycol (av mol wt, 6000) on the immunological reaction, albumin and fibrinogen were quantitated after a 10-min reaction time. Under the present conditions the sensitivity was increased and thus antiserum consumption was decreased. The reaction conditions were carefully studied with respect to optimal time and concentration of the reactants. The precision of the method was 2.6-3.6% (CV). The lower detection limit in the albumin-antialbumin system was 0.1 mg/liter. Comparative studies showed a correlation coefficient of 0.991 with an automated immunonephelometric method for urinary albumin, and of 0.908 with a thrombin clottable method for plasma fibrinogen. We especially suggest use of this manual immunonephelometric method where small series of samples are to be analyzed.