Kinetic evidence of the regeneration of multilineage hematopoiesis from primitive cells in normal human bone marrow transplanted into immunodeficient mice
Kinetic evidence of the regeneration of multilineage hematopoiesis from primitive cells in normal human bone marrow transplanted into immunodeficient mice
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DOI：
10.1182/blood.v89.12.4307
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发表时间：
1997-06-15
期刊：
影响因子：
20.3
通讯作者：
Eaves, CJ
中科院分区：
文献类型：
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作者：
Cashman, JD;Lapidot, T;Eaves, CJ
基于对一些经亚致死照射的严重联合免疫缺陷（SCID）小鼠静脉内移植正常人骨髓细胞的骨髓中人CD34（+）Thy-1（+）细胞和长期培养起始细胞（LTC-IC）的初步观察，以及随后发现NOD/LtSz-scid/scid（NOD/SCID）小鼠支持更高水平的人细胞植入，我们进行了一系列研究一系列时程实验旨在检查后一种小鼠模型中各种人类造血祖细胞群的数量、组织分布、循环活性和体内分化模式的移植后变化。这些研究显示了人 CD34(-) CD19(+) (B 系) 细胞、CD34(+) 粒细胞、红细胞和多系集落形成细胞 (CFC)、LTC-IC 和 CD34(+) Thy-1(+) 细胞的典型快速移植后恢复曲线，这些细胞来自一小部分初始群体，代表
Based on initial observations of human CD34(+) Thy-1(+) cells and long-term culture-initiating cells (LTC-IC) in the bone marrow of some sublethally irradiated severe combined immunodeficient (SCID) mice transplanted intravenously with normal human marrow cells, and the subsequent finding that the NOD/LtSz-scid/scid (NOD/SCID) mouse supports higher levels of human cell engraftment, we undertook a series of time course experiments to examine posttransplant changes in the number, tissue distribution, cycling activity, and in vivo differentiation pattern of various human hematopoietic progenitor cell populations in this latter mouse model. These studies showed typical rapid posttransplant recovery curves for human CD34(-) CD19(+) (B-lineage) cells, CD34(+) granulopoietic, erythroid, and multilineage colony-forming cells (CFC), LTC-IC, and CD34(+) Thy-1(+) cells from a small initial population representing