Purification and Characterization of a Protein from Porcine Gut with Glucagon-Like Immunoreactivity
Purification and Characterization of a Protein from Porcine Gut with Glucagon-Like Immunoreactivity
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具有胰高血糖素样免疫反应性的猪肠道蛋白的纯化和表征
DOI：
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发表时间：
1976
期刊：
影响因子：
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通讯作者：
Moody
中科院分区：
文献类型：
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作者：
F. Sundby;Jacobsen A.J;Moody
从猪肠中分离出一种具有胰高血糖素样免疫反应性的高纯度蛋白。等电点为6.8-6.9，氨基酸组成计算分子量为11,625，sds凝胶电泳证实了这一估计。目前已确定的部分序列为n端Arg-Ser-Leu-Gin-Asn-Thr-Glx-Glx-Lys-Ala-Arg-Ser-Phe-和c端- ile - ala，两者与猪胰高血糖素不同。在摩尔基础上，该蛋白与猪胰高血糖素具有相同的免疫反应性，当与某些抗胰高血糖素血清测定时，而与其他抗胰高血糖素血清测定时，活性低于0.2%。
A protein with glucagon-like immunoreactivity has been isolated from porcine intestine in a highly purified form. The isoelectric point is 6.8-6.9, and the molecular weight is 11,625, as calculated from its amino acid composition: this estimate has been confirmed by S.D.S. gel electrophoresis. The partial sequence so far elucidated is from the N-terminal: Arg-Ser-Leu-Gin-Asn-Thr-Glx-Glx-Lys-Ala-Arg-Ser-Phe-, and from the C-terminal: -Ile-Ala, both differing from those of porcine pancreatic glucagon. On a molar basis the protein has the same immunoreactivity as porcine glucagon when assayed with some anti-glucagon sera, while the activity is less than 0.2% using other anti-glucagon sera.