Fine Mapping of qPAA8, a Gene Controlling Panicle Apical Development in Rice
Fine Mapping of qPAA8, a Gene Controlling Panicle Apical Development in Rice
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qPAA8（一种控制水稻穗尖发育的基因）的精细定位
DOI：
10.1111/j.1744-7909.2011.01055.x
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发表时间：
2011-09-01
影响因子：
11.4
通讯作者：
Wan, Jianmin
中科院分区：
文献类型：
--
作者：
Cheng, Zhi-Jun;Mao, Bi-Gang;Wan, Jianmin
影响水稻单穗产量的一个不利因素是在不利的气候条件下频繁发生的穗尖败育（PAA）。到目前为止，还没有详细的遗传信息来避免水稻育种中的PAA。在这里，我们表明PAA的发生与过量过氧化氢的积累有关。在L-05261 （PAA系）与IRAT129（非PAA品种）杂交的F-2群体中，PAA的数量性状位点（qtl）定位鉴定出7个超过对数奇数阈值（LOD） 2.5的qtl，解释了PAA表型变异的约50.1%。从L-05261获得的5个效应增强的qtl分别为qPAA3-1、qPAA3-2、qPAA4、qPAA5和qPAA8，分别占表型变异的6.8%、5.9%、4.2%、13.0%和12.2%。结果表明，早抽穗植株的PAA主要受qPAA8控制。随后，利用基于标记辅助选择的qPAA8特异性亚群，我们进一步将qPAA8缩小到由标记RM22475和8-In112划分的37.6 kb区间。这些结果有利于PAA基因的克隆。
In rice, one detrimental factor influencing single panicle yield is the frequent occurrence of panicle apical abortion (PAA) under unfavorable climatic conditions. Until now, no detailed genetic information has been available to avoid PAA in rice breeding. Here, we show that the occurrence of PAA is associated with the accumulation of excess hydrogen peroxide. Quantitative trait loci (QTLs) mapping for PAA in an F-2 population derived from the cross of L-05261 (PAA line) x IRAT129 (non-PAA variety) identified seven QTLs over a logarithm of the odd (LOD) threshold of 2.5, explaining approximately 50.1% of phenotypic variance for PAA in total. Five of the QTLs with an increased effect from L-05261, were designated as qPAA3-1, qPAA3-2, qPAA4, qPAA5 and qPAA8, and accounted for 6.8%, 5.9%, 4.2%, 13.0% and 12.2% of phenotypic variance, respectively. We found that the PAA in the early heading plants was mainly controlled by qPAA8. Subsequently, using the sub-populations specific for qPAA8 based on marker-assisted selection, we further narrowed qPAA8 to a 37.6-kb interval delimited by markers RM22475 and 8-In112. These results are beneficial for PAA gene clone.