Steroid responses to ACTH-like polypeptides.
Steroid responses to ACTH-like polypeptides.
复制标题
对 ACTH 样多肽的类固醇反应。
DOI：
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发表时间：
1968
影响因子：
5.8
通讯作者：
J. Sunder
中科院分区：
文献类型：
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作者：
T. Danowski;K. Hofmann;F. Weigand;J. Sunder
在6小时内静脉给药75u纯化的猪ACTH，或商用ACTH，或0.75 mg合成ACTH样多肽，从n端表示该激素的氨基酸残基1-24、1-23或1-20，可产生相应的血浆11-(OH)皮质类固醇升高。因此，血浆皮质类固醇的增加所反映的促肾上腺皮质激素的充分活性表现为天然产物大小约为1 / 2的合成分子。此外，0.25 mg由24个氨基酸残基组成的多肽在延长6小时的静脉输注过程中被证明相当于75 U纯化的猪ACTH，可以提高血浆类固醇水平，这表明ACTH和较短的合成分子在摩尔基础上至少是等效的。一个合成的msh型分子，代表ACTH的前13个氨基酸残基，在人体内没有这种特性。氨基酸残基1-24的多肽剂量为0.75 mg时，血浆11-(OH) corti的升高幅度相同。
ABSTRACT The intravenous administration in 6 hr of 75 U of purified porcine ACTH, or of commercial ACTH or of 0.75 mg of a synthetic ACTH-like polypeptide representing, from the N-terminus, amino acid residues 1-24, 1-23, or 1-20 of this hormone produced comparable increases in plasma 11-(OH) corticosteroids. Hence, full corticotropin activity as reflected by increases in plasma corticosteroids was manifested by synthetic molecules about ½ the size of the natural product. Moreover, 0.25 mg of the polypeptide composed of 24 amino acid residues proved to be equal to 75 U of purified porcine ACTH in raising plasma steroids during a prolonged 6-hr intravenous infusion, establishing that ACTH and the shorter synthetic molecule are at least equipotent on a molar basis. A synthetic MSH-type molecule, representing the first 13 amino acid residues of ACTH, did not have this property in man. The 0.75 mg dosage of the polypeptide representing amino acid residues 1–24 yielded the same increase in plasma 11-(OH) corti...