Positive rate of RT-PCR detection of SARS-CoV-2 infection in 4880 cases from one hospital in Wuhan, China, from Jan to Feb 2020
Positive rate of RT-PCR detection of SARS-CoV-2 infection in 4880 cases from one hospital in Wuhan, China, from Jan to Feb 2020
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武汉市一院4880例SARS-CoV-2感染RT-PCR检测阳性率
DOI：
10.1016/j.cca.2020.03.009
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发表时间：
2020-06-01
影响因子：
5
通讯作者：
Zhu, Chengliang
中科院分区：
文献类型：
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作者：
Liu, Rui;Han, Huan;Zhu, Chengliang
背景资料：自2019年12月以来，新型冠状病毒（SARS-CoV-2）感染爆发，首先在中国，目前全球29个国家的确诊感染人数超过8万，直到2020年3月2日。早期识别、隔离和护理患者对于限制人际传播至关重要，包括减少密切接触者和医护人员的继发感染，防止传播放大事件。病毒核酸检测（NAT）的RT-PCR检测是新型病毒大流行中最快建立的实验室诊断方法之一，就像这次COVID-19爆发一样。方法：中国武汉4880例有呼吸道感染症状或与住院的COVID-19患者密切接触的病例，通过使用定量RT-PCR（qRT-PCR）对来自呼吸道的样本进行SARS-CoV-2感染检测。结果：4880份标本中，阳性率约为38%。男性和老年人的阳性率明显高于男性。然而，57%是阳性标本从发热诊所。二元Logistic回归分析显示，年龄是发热门诊SARS冠状病毒2型感染的危险因素，而非性别。结论：病毒NAT在SARS冠状病毒2型感染的诊断中起重要作用。
Background: There's an outbreak of a novel coronavirus (SARS-CoV-2) infection since December 2019, first in China, and currently with more than 80 thousand confirmed infection globally in 29 countries till March 2, 2020. Identification, isolation and caring for patients early are essential to limit human-to-human transmission including reducing secondary infections among close contacts and health care workers, preventing transmission amplification events. The RT-PCR detection of viral nucleic acid test (NAT) was one of the most quickly established laboratory diagnosis method in a novel viral pandemic, just as in this COVID-19 outbreak.Methods: 4880 cases that had respiratory infection symptoms or close contact with COVID-19 patients in hospital in Wuhan, China, were tested for SARS-CoV-2 infection by use of quantitative RT-PCR (qRT-PCR) on samples from the respiratory tract. Positive rates were calculated in groups divided by genders or ages.Results: The positive rate was about 38% for the total 4880 specimens. Male and older population had a significant higher positive rates. However, 57% was positive among the specimens from the Fever Clinics. Binary logistic regression analysis showed that age, not gender, was the risk factor for SARS-CoV-2 infection in fever clinics.Conclusions: Therefore, we concluded that viral NAT played an important role in identifying SARS-CoV-2 infection.