Microsecond time-scale discrimination among polycytidylic acid, polyadenylic acid, and polyuridylic acid as homopolymers or as segments within single RNA molecules
Microsecond time-scale discrimination among polycytidylic acid, polyadenylic acid, and polyuridylic acid as homopolymers or as segments within single RNA molecules
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DOI：
10.1016/s0006-3495(99)77153-5
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发表时间：
1999-12-01
影响因子：
3.4
通讯作者：
Deamer, DW
中科院分区：
文献类型：
--
作者：
Akeson, M;Branton, D;Deamer, DW
当DNA或RNA的单个分子被施加的电场驱动通过α-溶血素通道时，它们可以被检测到。在易位过程中，多核苷酸内的核苷酸必须以连续的单行顺序通过通道孔，因为孔的极限直径一次只能容纳一条DNA或RNA链。在这里，我们证明了这种纳米孔的行为作为一个检测器，可以快速区分嘧啶和嘌呤片段沿着RNA分子。单分子的纳米孔检测和表征代表了直接阅读线性聚合物中编码的信息的新方法，并且是直接测序单个DNA和RNA分子的关键第一步。
Single molecules of DNA or RNA can be detected as they are driven through an oc-hemolysin channel by an applied electric field. During translocation, nucleotides within the polynucleotide must pass through the channel pore in sequential, single-file order because the limiting diameter of the pore can accommodate only one strand of DNA or RNA at a time. Here we demonstrate that this nanopore behaves as a detector that can rapidly discriminate between pyrimidine and purine segments along an RNA molecule. Nanopore detection and characterization of single molecules represent a new method for directly reading information encoded in linear polymers, and are critical first steps toward direct sequencing of individual DNA and RNA molecules.