Enhanced melatonin production via aralkylamine N-acetyltransferase overexpression enhances NaCl resistance in transgenic Chlamydomonas reinhardtii (Volvocales, Chlorophyceae)
Enhanced melatonin production via aralkylamine N-acetyltransferase overexpression enhances NaCl resistance in transgenic Chlamydomonas reinhardtii (Volvocales, Chlorophyceae)
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通过芳烷基胺 N-乙酰转移酶过度表达增强褪黑激素的产生可增强转基因莱因衣藻（藻类、绿藻纲）的 NaCl 抗性
DOI：
10.1080/00318884.2018.1529971
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发表时间：
2019-03
期刊：
影响因子：
1.6
通讯作者：
Wang Yingjuan
中科院分区：
文献类型：
--
作者：
Zhang Yujing;Gao Wenying;Lv Yawei;Bai Qingqing;Wang Yingjuan
摘要褪黑激素（MT）是由动物的松果体分泌的，也存在于植物和藻类中。MT不仅调节睡眠，减少时差的影响，在动物中具有抗衰老特性，而且还消除细胞内活性氧，促进植物的营养生长。然而，关于其在微藻中的生物学功能的研究还很少。本研究构建了莱茵衣藻表达载体pDBle-AANAT，该载体含有MT合成途径中的一种酶芳烷基胺N-乙酰转移酶（AANAT）的基因。将AANAT基因导入到模式菌株C的基因组中。reinhardtii通过玻璃珠转化。通过抗性筛选、培养和分子生物学方法对转AANAT基因藻株进行了鉴定。检测了转化藻株和未转化藻株MT含量的变化，并分析了AANAT过表达藻株在盐胁迫下的抗氧化能力。结果表明，转基因藻可以通过过量表达AANAT来提高藻细胞中MT的含量。在盐胁迫条件下，这有助于提高藻细胞抗氧化系统的酶活性，减少膜损伤，以对抗不利的外部胁迫。这增强了C.莱因哈德氏菌本研究揭示了MT过量生产对C.莱因哈德氏菌此外，还探讨了MT在C.已阐明了MT对植物胁迫的作用，从而拓宽了MT在植物中的功能范围。
ABSTRACT The hormone melatonin (MT) is produced by the pineal gland in animals and can also be found in plants and algae. MT regulates not only sleep, reduces the effects of jet lag, and has anti-aging properties in animals, but also eliminates intracellular reactive oxygen species and promotes vegetative growth in plants. However, research on its biological function in microalgae is still rare. The present study involved the construction of the Chlamydomonas reinhardtii expression vector pDBle-AANAT, which harbours the gene of one of the enzymes of the MT synthesis pathway, aralkylamine N-acetyltransferase (AANAT). The AANAT gene was introduced into the genome of the model microalga C. reinhardtii via glass bead transformation. The AANAT transgenic algal strain was identified by resistance screening, culture, and molecular biology methods. Changes in MT content between transformed algal strain and nontransformed algal strains were detected and the antioxidant capacity of the AANAT overexpressing algal strain was analysed under salt stress. The results showed that genetically engineered algae can increase the content of MT in algal cells by overexpressing AANAT. Under salt stress conditions, this can help to improve the enzymatic activity of the antioxidant system of algal cells and reduce membrane damage to counteract the adverse external stress. This enhances the self-protection ability of C. reinhardtii. This study reveals the effects of MT overproduction on the physiological functions of C. reinhardtii. Additionally, the potential functions of MT in the response of C. reinhardtii to stress have been elucidated, thus broadening the functional scope of MT in plants.
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DOI：
--
发表时间：
2012
期刊：
Acta Pratacultural Science
影响因子：
--
作者：
Jiang Hai-dong
通讯作者：
Jiang Hai-dong
影响因子：
5.6
作者：
Carrillo-Vico A;Lardone PJ;Alvarez-Sánchez N;Rodríguez-Rodríguez A;Guerrero JM
通讯作者：
Guerrero JM
DOI：
10.1007/978-1-61779-986-0
发表时间：
2012
期刊：
--
影响因子：
--
作者：
S. Shabala;T. Cuin
通讯作者：
S. Shabala;T. Cuin
影响因子：
64.8
作者：
J. Ellis
通讯作者：
J. Ellis
DOI：
10.1007/978-3-540-39402-0_10
发表时间：
2004-07
期刊：
--
影响因子：
--
作者：
V. Chinnusamy;Jian-Kang Zhu
通讯作者：
V. Chinnusamy;Jian-Kang Zhu