Vibrio ouci sp. nov. and Vibrio aquaticus sp. nov., two marine bacteria isolated from the East China Sea
Vibrio ouci sp. nov. and Vibrio aquaticus sp. nov., two marine bacteria isolated from the East China Sea
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欧西弧菌
DOI：
10.1099/ijsem.0.003732
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发表时间：
2020
影响因子：
2.8
通讯作者：
Zhang Xiao-Hua
中科院分区：
文献类型：
--
作者：
Li Bei;Li Yuying;Liu Ronghua;Xue Chunxu;Zhu Xiaoyu;Tian Xiaorong;Wang Xiaolei;Liang Jinchang;Zheng Yanfen;Zhang Xiao-Hua
从东海海水中分离到2株革兰氏染色阴性、过氧化氢酶和氧化酶阳性、兼性厌氧、杆状运动细菌，命名为BEI 176 T和BEI 207 T。16 S rRNA基因序列分析表明，菌株BEI 176 T和BEI 207 T均属于弧菌属，两者的亲缘关系最近，相似性为98.18%。 菌株BEI 176 T与溶藻弧菌LMG 4409 T（98.85%）和坎氏弧菌LMG 11216 T（98.81%）的亲缘关系最近，而菌株BEI 207 Twa与肝弧菌LMG 20362 T（98.64%）的亲缘关系最近。 这两种菌株在不同的条件下显示出生长;而菌株BEI 176 T在16-37 °C，pH 5.0-9.5和0- 7.0%（w/v）NaCl下生长，而菌株BEI 207 T在10-37 °C，pH 6.0-9.5和1.0- 7.0%（w/v）NaCl下生长。 这两个菌株具有相同的总特征3（C16：1ω 7 cor C16：1ω6c）、C16：0和总特征8（C18：1ω6cor C18：1ω 7 c）的主要脂肪酸组分。 BEI 176 T和BEI 207 T的DNA G+C含量分别为44.73mol%和45.06mol%。两个菌株与其参考种之间的平均核苷酸同一性值低于种划分的阈值（95- 96%）;计算机DNA-DNA杂交进一步表明，两个菌株与其亲缘种的相似性低于70%。 因此，提出了两个新的弧菌种来容纳它们：弧菌oucisp。nov.（模式菌株BEI 176 T =MCCC 1 K 03515 T =JCM 32690 T = KCTC 62616 T）和Vibrioaquaticussp. nov.（模式菌株BEI 207 T =MCCC 1 K 03516 T =JCM 32691 T =KCTC 62617 T）。
Two Gram-stain-negative, catalase- and oxidase-positive, facultative anaerobic and rod-shaped motile bacteria, designated strains BEI176Tand BEI207T, were isolated from seawater collected in the East China Sea. Phylogenetic analysis based on 16S rRNA gene sequences showed that strains BEI176Tand BEI207Tbelonged to the genusVibrioand were closely related to each other with 98.18 % similarity. The closest phylogenetic relatives of strain BEI176TwereVibrio alginolyticusLMG 4409T(98.85 %) andVibrio campbelliiLMG 11216T(98.81 %), whereas the closest relative of strain BEI207TwasVibrio hepatariusLMG 20362T(98.64 %). The two strains showed growth at different conditions; while strain BEI176Tgrew at 16–37 °C, pH 5.0–9.5 and 0–7.0 % (w/v) NaCl, the growth of strain BEI207Toccurred at 10–37 °C, pH 6.0–9.5 and 1.0–7.0 % (w/v) NaCl. Both strains shared the same major fatty acid components of summed feature 3 (C16 : 1ω7cor C16 : 1ω6c), C16 : 0and summed feature 8 (C18 : 1ω6cor C18 : 1ω7c). The DNA G+C contents of the assembled genomic sequences were 44.73 and 45.06 mol% for strains BEI176Tand BEI207T, respectively. Average nucleotide identity values between the two strains and their reference species were lower than the threshold for species delineation (95–96 %);in silicoDNA–DNA hybridization further showed that the two strains had less than 70 % similarity to their relatives. Therefore, two novelVibriospecies are proposed to accommodate them:Vibrio oucisp. nov. (type strain, BEI176T=MCCC 1K03515T=JCM 32690T= KCTC 62616T) andVibrio aquaticussp. nov. (type strain, BEI207T=MCCC 1K03516T=JCM 32691T=KCTC 62617T).