Bio rapid prototyping by extruding/aspirating/refilling thermoreversible hydrogel
Bio rapid prototyping by extruding/aspirating/refilling thermoreversible hydrogel
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DOI：
10.1088/1758-5082/2/1/014108
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发表时间：
2010-03-01
期刊：
影响因子：
9
通讯作者：
Umeda, N.
中科院分区：
文献类型：
--
作者：
Iwami, K.;Noda, T.;Umeda, N.
本文报道了一种用于细胞组织快速成型的方法，该方法基于一种挤压、吸入和重新填充细胞和热可逆水凝胶的混合物作为支架的系统。在挤压模式中，将处于溶胶态的细胞混合支架溶液从冷却的微喷嘴挤出到受控温度的衬底中，从而使支架保持在凝胶状态。在抽吸模式下，相反的过程是通过伯努利抽吸进行的。在重新灌装模式下，溶液被挤出到在吸入模式下形成的凹槽中。采用直径为100微米的喷嘴时，挤出水凝胶图案的最小宽度为114±15微米；采用直径为500微米的喷嘴时，吸气槽的最小宽度为355±10微米。阿拉伯树胶与支架溶液混合，以避免凝胶图案从基材上剥落。展示了SF-9细胞组织的图案化，并研究了图案化细胞的稳定性。该系统为组织工程细胞支架的快速成型和局部修饰提供了一种方法。
This paper reports a method for rapid prototyping of cell tissues, which is based on a system that extrudes, aspirates and refills a mixture of cells and thermoreversible hydrogel as a scaffold. In the extruding mode, a cell-mixed scaffold solution in the sol state is extruded from a cooled micronozzle into a temperature-controlled substrate, which keeps the scaffold in the gel state. In the aspiration mode, the opposite process is performed by Bernoulli suction. In the refilling mode, the solution is extruded into a groove created in the aspiration mode. The minimum width of extruded hydrogel pattern is 114 +/- 15 mu m by employing a nozzle of diameter 100 mu m, and that of aspirated groove was 355 +/- 10 mu m using a 500 mu m-diameter nozzle. Gum arabic is mixed with the scaffold solution to avoid peeling-off of the gel pattern from the substrate. Patterning of Sf-9 cell tissue is demonstrated, and the stability of the patterned cell is investigated. This system offers a procedure for rapid prototyping and local modification of cell scaffolds for tissue engineering.