Distinct recognition phenotypes exist for T cell clones specific for small peptide regions of proteins. Implications for the mechanisms underlying major histocompatibility complex-restricted antigen recognition and clonal deletion models of immune response gene defects.
Distinct recognition phenotypes exist for T cell clones specific for small peptide regions of proteins. Implications for the mechanisms underlying major histocompatibility complex-restricted antigen recognition and clonal deletion models of immune response gene defects.
复制标题
针对特异性的蛋白质小肽区域的T细胞克隆存在明显的识别表型。对主要的组织相容性抗原识别和免疫反应基因缺陷的克隆缺失模型的基础机制的影响。
DOI：
10.1084/jem.162.1.332
复制
发表时间：
1985-07-01
期刊：
影响因子：
--
通讯作者：
Sercarz EE
中科院分区：
文献类型：
--
作者：
Shastri N;Oki A;Miller A;Sercarz EE
使用合成肽作为抗原，发现特定于13-16个氨基酸肽的给定单倍型的T细胞克隆可以通过氨基酸取代对识别的不同影响而清楚地区分。这是真实的不同的抗原决定簇内的肽81-96和74-86的鸡蛋白溶菌酶，承认在上下文中的I-Ab和I-Ak分子，分别。在对明显单一决定簇特异的诱导T细胞库中证明了相当大的复杂性，这意味着T细胞识别的多样性接近于B细胞。退化的T细胞识别的影响进行了讨论的背景下，通过Ia分子限制识别和理论的Ir基因缺陷的机制。
Using synthetic peptides as antigens, it was found that T cell clones of a given haplotype specific for 13-16 amino acid peptides could be clearly distinguished by the varied influence of amino acid substitutions on recognition. This was true for different antigenic determinants within peptides 81-96 and 74-86 of hen egg-white lysozyme, recognized in the context of the I-Ab and I-Ak molecules, respectively. Considerable complexity was demonstrated in the induced T cell repertoire specific for apparently single determinants, which implies that diversity of T cell recognition approaches that for B cells. The implications of the degeneracy of T cell recognition are discussed in the context of mechanisms through which Ia molecules restrict recognition and theories of Ir gene defects.