Comparison of the resistance to sympatric and allopatric isolates of Haemonchus contortus of Black Belly sheep in Guadeloupe (FWI) and of INRA 401 sheep in France
Comparison of the resistance to sympatric and allopatric isolates of Haemonchus contortus of Black Belly sheep in Guadeloupe (FWI) and of INRA 401 sheep in France
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DOI：
10.1016/s0304-4017(03)00259-0
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发表时间：
2003-10-08
影响因子：
2.6
通讯作者：
Hostache, G
中科院分区：
文献类型：
--
作者：
Aumont, G;Gruner, L;Hostache, G
按照2 x 2 x 2实验设计，将热带绵羊品种对线虫寄生虫捻转血矛线虫（He）的抗性与人工感染寄生虫后的欧洲品种进行比较：两个分离物（来自瓜德罗普的Hc Gua和来自法国的He Fra），两个品种均在其本土栖息地饲养（瓜德罗普的黑腹（BB），FWI，在法国饲养的INRA 401）和两组感染状况不同的羔羊（一只未感染，即在实验期间第一次感染，一只致敏，即，在本实验期间，在感染前的第-35天和第-32天先前接受了5000 L3的剂量）。两组均由10只雄性羔羊组成，在第0天给予10，000 L3剂量的相关分离株时为20周龄。通过定期粪便虫卵计数（FEC）和血液样本评估抗性，并在感染后第35天处理蠕虫计数。BB中的FEC低于INRA 401羔羊，致敏组低于未致敏组，He Fra低于He Gua。在INRA 401初治组中，嗜酸性粒细胞计数保持不变，但在所有其他组中增加。感染后红细胞压积下降，但活重不受感染的影响。未处理BB组和INRA 401组的蠕虫负荷分别为1868和5237，预处理BB组和INRA 401组分别为596和4835。每头鹤瓜雌虫的日产卵量是Hc Fra的1.5倍。在BB绵羊中，蠕虫的数量对蠕虫长度或子宫内卵计数没有影响，但在INRA 401羔羊中观察到这两个性状之间的正相关和显著相关。这些发现表明先天性抗性，并证实了高获得性抗性的BB羊的同域和异域分离株的HC。BB和INRA 401绵羊对蠕虫种群和繁殖力的调节不同。(C)2003 Elsevier B. V.保留所有权利。
The resistance of a tropical breed of sheep towards the nematode parasite Haemonchus contortus (He) was compared to that of a European breed after artificial infection with the parasite following a 2 x 2 x 2 experimental design: two isolates (Hc Gua from Guadeloupe versus He Fra from France), two breeds each reared in their native habitats (Black Belly (BB) in Guadeloupe, FWI, INRA 401 reared in France), and two groups of lambs with differing infection status (one naive, i.e. infected for the first time during the experiment, and one primed, i.e. which had previously received doses of 5000 L3 on Days -35 and -32 before the infection during this experiment). Both groups were composed of 10 male lambs, which were 20 weeks of age when they were given a dose of 10,000 L3 of the relevant isolate on Day 0. Resistance was assessed by regular fecal egg counts (FECs) and blood samples, and worm counts were processed on Day 35 p.i.The FEC was lower in the BB than in INRA 401 lambs, lower in the primed than naive groups, and lower in He Fra than in He Gua. The eosinophil count remained unchanged in the naive INRA 401 groups, but increased in all the other groups. The hematocrit fell after infection, but the live weight was not affected by the infection. Worm burdens were 1868 and 5237 in the naive BB and INRA 401 groups, respectively, and 596 and 4835 in the primed BB and INRA 401 groups. The daily production of eggs per He Gua female worm was 1.5-fold that of Hc Fra. The number of worms had no effect on worm length or He in utero egg count in the BB sheep, but a positive and significant correlation between these two traits was seen in the INRA 401 lambs. These findings are indicative of innate resistance, and confirmed the high acquired resistance of the BB sheep to both sympatric and allopatric isolates of Hc. The regulation of worm population and fecundity differed in the BB and INRA 401 sheep. (C) 2003 Elsevier B.V. All rights reserved.