A response surface approach for the comparison of lipase production by Candida cylindracea using two different carbon sources
A response surface approach for the comparison of lipase production by Candida cylindracea using two different carbon sources
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DOI：
10.1016/s1369-703x(01)00117-6
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发表时间：
2001-11-01
影响因子：
3.9
通讯作者：
Nigam, P
中科院分区：
文献类型：
--
作者：
Muralidhar, RV;Chirumamila, RR;Nigam, P
采用响应面法对圆柱假丝酵母产胞外脂肪酶进行了研究。通过中心组合设计优化了脂肪酶生产的培养基成分。在实验中使用2（5）设计矩阵，仅改变碳源。以葡萄糖和橄榄油为碳源时，脂肪酶的最适产率分别为17.30 U/ml和47.25 U/ml。同时优化了所有其他培养基成分，即酵母提取物、麦芽提取物、蛋白胨和吐温80。在预测的最佳水平下进行的实验与预测的最佳值吻合。在此实验条件下，橄榄油培养基是较好的发酵培养基。柱孢菌脂肪酶(C)2001 Elsevier Science B. V.保留所有权利。
A response surface approach has been used to study the production of extracellular lipase by Candida cylindracea. Medium constituents were optimised for the production of lipase by central composite design. A 2(5) design matrix was used in the experiments with only the carbon source being varied. The optimal yield of lipase was 17.30 U/ml using glucose as the carbon source and 47.25 U/ml using olive oil as the carbon source. All the other medium constituents namely yeast extract, malt extract, peptone and tween 80 were simultaneously optimised. Experiments conducted at the predicted optimum levels fitted with the predicted optima. Based on these experimental conditions, medium containing olive oil appears to a better medium for fermentative production of C. cylindracea lipase. (C) 2001 Elsevier Science B.V. All rights reserved.