CELL CELL COMMUNICATION IN THE EMBRYO OF CAENORHABDITIS-ELEGANS
CELL CELL COMMUNICATION IN THE EMBRYO OF CAENORHABDITIS-ELEGANS
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DOI：
10.1016/0012-1606(92)90180-o
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发表时间：
1992-06-01
影响因子：
2.7
通讯作者：
SCHIERENBERG, E
中科院分区：
文献类型：
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作者：
BOSSINGER, O;SCHIERENBERG, E
我们研究了自由生活土壤线虫（decaenorhabditis elegans）胚胎中细胞-细胞通讯的模式。为此，我们建立了在单个卵裂球中微量注射示踪染料的方法。不同分子量的荧光染料（路西法黄，LY,Mr457；罗丹明标记的葡聚糖，RD,Mr4000）离子电泳后，我们可以看到细胞间的通讯途径。LY的染色扩散，表明通过间隙连接进行通信，在2细胞晚期首次可见。从4细胞阶段开始，所有细胞似乎都通过通信通道很好地耦合，这允许LY的自由扩散。相反，RD仍然局限于注射细胞及其后代。在24细胞期产生原始生殖细胞p4后，LY向该细胞及其体细胞姐妹细胞D的染料扩散被延迟。然而，D的受限染料偶联只是暂时的。经过一段时间后，它加入了体细胞隔室。随着形态发生阶段的开始，两个现有的生殖系细胞（P4的子细胞）与体细胞完全分离，而后者仍然形成一个单一的染料偶联室。只有在胚胎发生的后半期，不同的独立的体细胞通讯室才建立起来。我们跟踪了消化道细胞间通讯的模式，发现了一个逐渐限制到更小的染料偶联单位。我们的数据与其他系统中发现的数据进行了比较，并讨论了关于细胞测定和分化的问题。
We have investigated the pattern of cell-cell communication in embryos of the free-living soil nematodeCaenorhabditis elegans. For this, we have established a method for microinjection of tracer dyes into individual blastomeres. After iontophoresis of fluorescent dyes of different molecular weights (Lucifer yellow, LY,Mr457; rhodamine-labeled dextran, RD,Mr4000), we can visualize intercellular communication pathways. The dye-spread of LY, indicating communication via gap junctions, becomes first visible in the late 2-cell stage. From the 4-cell stage onward all cells appear to be well coupled by communication channels, which allow the free diffusion of LY. In contrast, RD remains restricted to the injected cell and its descendants. After the primordial germcell P4has been generated in the 24-cell stage, dye-spread of LY into this cell and its somatic sister D is delayed. However, the restricted dye-coupling of D is only temporary. After a brief period it joins the somatic compartment. With the beginning of the morphogenesis phase the two existing germline cells (the daughters of P4) are completely uncoupled from the soma, while the latter still forms a single dye-coupling compartment. Only during the second half of embryogenesis different separate somatic communication compartments are established. We followed the pattern of intercellular communication in the alimentary tract and found a progressive restriction into smaller dye-coupling units. Our data are compared to those found in other systems and discussed with respect to cellular determination and differentiation.