Mild prenatal stress in rats is associated with enhanced conditioned fear
Mild prenatal stress in rats is associated with enhanced conditioned fear
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DOI：
10.1016/s0031-9384(03)00097-0
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发表时间：
2003-07-01
影响因子：
2.9
通讯作者：
Birkle, DL
中科院分区：
文献类型：
--
作者：
Griffin, WC;Skinner, HD;Birkle, DL
我们测试了这样的假设：产前压力会增强成年大鼠的条件性恐惧。从怀孕第 14 天到第 21 天，怀孕的 Sprague-Dawley 大鼠通过暴露于新的环境和每天随机时间皮下注射盐水（0.1 ml 0.9% NaCl）来承受应激。与来自未受操作妊娠的成年对照（CON）雄性大鼠相比，成年产前应激（PS）雄性大鼠表现出对急性足部电击的反应增加的僵直行为，以及第二天在相同情况下（没有电击）的僵直行为增加。在另一项实验中，检查了妊娠应激源中皮质酮和 ACTH 的升高情况。在妊娠天 (G)15、G17、G19 和 G21 时，收集母体和胎儿血浆。分析显示，与 CON 水坝相比，PS 水坝的皮质酮和 ACTH 有所升高。此外，与 CON 胎儿相比，PS 胎儿的皮质酮水平有所增加。最后，检查了一些 CON 和 PS 窝的妊娠时长、出生幼仔数量、出生后体重 (P)1 和 P1 肛门生殖器距离的变化，但没有发现差异。总之，我们的数据表明，妊娠期间的轻微压力足以提高血浆皮质酮和促肾上腺皮质激素，与成年期条件性恐惧的增强有关。 (C) 2003 Elsevier Science Inc. 保留所有权利。
We tested the hypothesis that prenatal stress would enhance conditioned fear in adult rats. Pregnant Sprague-Dawley rats were stressed by exposure to a novel environment and subcutaneous injection of saline (0.1 ml 0.9% NaCl) at random times daily from Days 14 to 21 of pregnancy. When compared to adult control (CON) male rats from unmanipulated pregnancies, adult prenatally stressed (PS) male rats showed increased freezing behavior in response to acute footshock as well as increased freezing behavior the next day in the same context, without shock delivery. In another experiment, the gestational stressor was examined for elevations in corticosterone and ACTH. At gestational days (G)15, G17, G19 and G21, maternal and fetal plasma was collected. Analysis showed elevations in corticosterone and ACTH in the PS dams when compared to the CON dams. Additionally, increased corticosterone was found in the PS fetuses when compared to the CON fetuses. Finally, some CON and PS litters were examined for alterations in length of gestation, number of pups born, bodyweight on postnatal day (P)1 and anogenital distance on P1 and differences were not found. In conclusion, our data demonstrate that a mild stressor during gestation, sufficient to raise plasma corticosterome and ACTH, is associated with enhanced conditioned fear during adulthood. (C) 2003 Elsevier Science Inc. All rights reserved.