Synergistic effects of titania nanotubes and silicon to enhance the osteogenic activity.
Synergistic effects of titania nanotubes and silicon to enhance the osteogenic activity.
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DOI：
10.1016/j.colsurfb.2018.07.052
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发表时间：
2018-11
期刊：
影响因子：
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通讯作者：
Tao Wang;Shi Qian;Guo‐chun Zha;Xijiang Zhao;L. Ding;Junying Sun;Bin Li;Xuanyong Liu
中科院分区：
文献类型：
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作者：
Tao Wang;Shi Qian;Guo‐chun Zha;Xijiang Zhao;L. Ding;Junying Sun;Bin Li;Xuanyong Liu
在这项研究中，二氧化钛纳米管（TNTs）纳入硅（Si）的钛盘形成使用阳极氧化和电子束蒸发（EBE）技术，以提高成骨活性。通过控制EBE的持续时间来精细地调节Si的量以优化生物功能性。随着Si的掺入，样品表现出亲水性表面。从EBE改性的样品中观察到持久和可控的Si释放，而没有细胞毒性。此外，对MC 3 T3-E1细胞的初始细胞粘附、铺展、增殖和成骨分化进行了评价。结果表明，硅涂层的TNTs（Si-TNTs）上的细胞的铺展，成骨和分化的显着增强。特别是，与本研究中的其他样品相比，硅含量最高的样品（约5.93%Si）显示出ALP活性、成骨相关基因表达和矿化的最大增强。结果表明，TNTs和适量的Si改性可促进成骨细胞的铺展、增殖和分化，在骨科领域具有潜在的应用前景。
In this study, titania nanotubes (TNTs) incorporating silicon (Si) were formed on Ti disks using anodization and electron beam evaporation (EBE) technology to improve the osteogenic activity. The amount of Si was exquisitely adjusted by controlling the duration of EBE to optimize the biofunctionality. As the Si was incorporated, the samples exhibited hydrophilic surfaces. Long lasting and controllable Si release was observed from the EBE-modified samples without cytotoxicity. Moreover, initial cell adhesion, spreading, proliferation and osteogenic differentiation of MC3T3-E1 cells were evaluated. The results showed a notable enhancement of spreading, osteogenesis and differentiation of cells on silicon-coated TNTs (Si-TNTs). In particular, samples with highest amount of silicon (∼5.93% Si) displayed greatest augmentation of ALP activity, osteogenic-related gene expression and mineralization compared to the others in the present study. It was indicated that the modification with TNTs and appropriated Si content resulted in enhanced osteoblastic spreading, proliferation and differentiation, and therefore has the potential for future applications in the field of orthopedics.