Immigration and emigration of Burkholderia cepacia and Pseudomonas aeruginosa between and within mixed biofilm communities
Immigration and emigration of Burkholderia cepacia and Pseudomonas aeruginosa between and within mixed biofilm communities
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DOI：
10.1111/j.1365-2672.2004.02201.x
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发表时间：
2004-01-01
影响因子：
4
通讯作者：
Allison, DG
中科院分区：
文献类型：
--
作者：
Al-Bakri, AG;Gilbert, P;Allison, DG
目的：通过使用生物膜生长的Sorbarod模型和恒定深度膜发酵罐（CDFF）来研究二元培养生物膜形成的动力学。方法和结果：铜绿假单胞菌的实验室和临床菌株的假稳态生物膜培养物，基于它们产生洋葱伯克霍尔德氏菌生长抑制物质的能力进行选择，在Sorbarod过滤器上建立，并用相应的B的增殖生长培养物进行攻击。洋葱。还进行了反向挑战。都是B。洋葱和铜绿假单胞菌能够在48小时生长后形成稳定状态的单一培养物生物膜。当B.洋葱NTCT 10661用已知产生B的培养的铜绿假单胞菌PAO 1攻击。洋葱生长抑制物质，移民人口迅速和几乎完全结合到生物膜，取代B。洋葱。相比之下，铜绿假单胞菌PAO 1的已建立的生物膜抵抗B的迁移。洋葱10661。使用产生铜绿假单胞菌313113和B临床配对的非生长抑制物质进行类似实验。洋葱313113导致在两种情况下形成稳定的、混合的生物膜群体。此外，与这些临床分离株共接种导致稳定的混合稳态生物膜。对CDFF中产生的生物膜进行了类似的观察。在这种情况下，在两个单一培养物CDFF之间进行泛交换后，B.洋葱313113能够整合到已建立的铜绿假单胞菌313113生物膜中以形成稳定的二元生物膜。结论：混合物种群落的建立遵循特定的接种顺序，这可能是由于共同定殖者之间的某种程度的匹配，或者铜绿假单胞菌倾向于允许B的表面的未定殖部分。该研究的意义和影响：一种细菌物种在表面的定殖赋予了对其他物种的定殖抗性。表面消毒可能会增加病原体藏匿的可能性。
Aims: To investigate the dynamics of binary culture biofilm formation through use of both the Sorbarod model of biofilm growth and the constant depth film fermenter (CDFF).Methods and Results: Pseudo steady-state biofilm cultures of laboratory and clinical strains of Pseudomonas aeruginosa, selected on the basis of their ability to produce a Burkholderia cepacia growth-inhibitory substance, were established on Sorbarod filters and challenged with corresponding planktonic grown cultures of B. cepacia. Reverse challenges were also conducted. Both B. cepacia and P. aeruginosa were able to form steady-state monoculture biofilms after 48 h growth. When steady-state biofilms of B. cepacia NTCT 10661 were challenged with planktonically grown P. aeruginosa PAO1 known to produce a B. cepacia growth-inhibitory substance, the immigrant population was rapidly and almost completely bound to the biofilm, displacing B. cepacia. By contrast, established biofilms of P. aeruginosa PAO1 resisted immigration of B. cepacia 10661. Similar experiments conducted with a nongrowth inhibitory substance producing clinical pairing of P. aeruginosa 313113 and B. cepacia 313113 led to the formation of stable, mixed biofilm populations in both instances. Moreover, co-inoculation with these clinical isolates resulted in a stable, mixed steady-state biofilm. Similar observations were made for biofilms generated in CDFFs. In such instances following pan-swapping between two monoculture CDFFs, B. cepacia 313113 was able to integrate into an established P. aeruginosa 313113 biofilm to form a stable binary biofilm.Conclusions: Establishment of a mixed species community follows a specific sequence of inoculation that may either be due to some degree of match between co-colonizers or that P. aeruginosa predisposes uncolonized sections of the surface to permit B. cepacia colonization.Significance and Impact of the Study: Colonization of a surface with one bacterial species confers colonization resistance towards other species. Disinfection of a surface might well increase the probability of pathogen harbourage.