IMMUNOCOMPETENCE AND OXIDANT DEFENSE DURING ASCORBATE DEPLETION OF HEALTHY-MEN
IMMUNOCOMPETENCE AND OXIDANT DEFENSE DURING ASCORBATE DEPLETION OF HEALTHY-MEN
复制标题
DOI：
10.1093/ajcn/54.6.1302s
复制
发表时间：
1991-12-01
影响因子：
7.1
通讯作者：
PETERS, JH
中科院分区：
文献类型：
--
作者：
JACOB, RA;KELLEY, DS;PETERS, JH
为了确定中度维生素C缺乏对非坏血病的影响，我们测量了8名健康男性（25-43岁）的免疫功能和氧化损伤，他们在代谢单位上摄入5-250 mg/d的抗坏血酸超过92天。在5、10或20 mg/d的抗坏血酸摄入量期间，受试者达到中度抗坏血酸缺乏状态，因为血浆、白细胞、精液和口腔细胞中的抗坏血酸浓度降至基线的50%以下，未观察到坏血病症状。未观察到细胞增殖、红细胞抗氧化酶和DNA链断裂的变化;然而，在抗坏血酸缺乏期间，谷胱甘肽和NAD（P）的血液水平降低，迟发型超敏反应性也降低。氧化修饰的DNA碱基，8-羟基脱氧鸟苷在精子DNA和fecapentaenes，无处不在的粪便诱变剂的浓度，在抗坏血酸消耗增加。在没有坏血病的情况下，中度维生素C缺乏会导致抗氧化化学物质的改变，并可能增加氧化损伤。
To determine nonscorbutic effects of moderate vitamin C deficiency we measured immune function and oxidative damage in eight healthy men (25-43 y) who consumed 5-250 mg/d of ascorbic acid over 92 d on a metabolic unit. During ascorbic acid intakes of 5, 10, or 20 mg/d, subjects attained a state of moderate ascorbic acid deficiency as ascorbic acid concentrations in plasma, leucocytes, semen, and buccal cells dropped to < 50% of baseline with no scorbutic symptoms observed. No changes in cell proliferation, erythrocyte antioxidant enzymes, and DNA strand breaks were observed; however, blood levels of glutathione and NAD(P) decreased during ascorbic acid deficiency, as did delayed hypersensitivity responsiveness. Concentrations of the oxidatively modified DNA base, 8-hydroxydeoxyguanosine in sperm DNA and fecapentaenes, ubiquitous fecal mutagens, were increased during ascorbic acid depletion. Moderate vitamin C deficiency, in the absence of scurvy, results in alteration of antioxidant chemistries and may permit increased oxidative damage.