Zebrafish intelectin 1 (zITLN1) plays a role in the innate immune response
Zebrafish intelectin 1 (zITLN1) plays a role in the innate immune response
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斑马鱼智力素 1 (zITLN1) 在先天免疫反应中发挥作用
DOI：
10.1016/j.fsi.2018.09.004
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发表时间：
2018-12-01
影响因子：
4.7
通讯作者：
Feng, Lijun
中科院分区：
文献类型：
--
作者：
Chen, Lei;Yan, Jie;Feng, Lijun
Inteltin显示出碳水化合物结合能力，并已被证明能凝集细菌，表明其在先天性免疫中的作用。它还被认为与人类的许多致病疾病有关。在报道了两个文昌鱼的同源基因和斑马鱼的内毒素2(ZITLN1)之后，我们克隆并鉴定了斑马鱼的内毒素1(ZITLN1)。与zITLN2类似，zITLN1还含有一个保守的纤维蛋白原相关结构域(FRED)和一个独特的intelectin结构域(ITLN-D)，在所有受试组织中都有表达，其中以肠道表达最高，并在急性期对细菌攻击做出反应。我们还在大肠杆菌系统中表达了zITLN1，纯化的重组zITLN1能以钙依赖的方式凝集革兰氏阳性菌和革兰氏阴性菌。它对革兰氏阳性菌的凝集能力强于对革兰氏阴性菌的凝集能力，而zITLN2对革兰氏阳性菌没有这种偏好。这可能是因为重组的zITLN1能较高程度地与脂多糖结合。我们对zITLN1的研究结果为研究intelectin家族的进化和功能提供了新的视角。
Intelectin displays carbohydrate binding capacity and has been demonstrated to agglutinate bacteria, suggesting its role in innate immunity. It has also been linked to many pathogenic conditions in human. After reporting two amphioxus orthologs and the zebrafish intelectin 2 (zITLN2), here we cloned and characterized zebrafish intelectin 1 (zITLN1). Like zITLN2, zITLN1 also contains a conserved fibrinogen-related domain (FReD) and a unique intelectin domain (ITLN-D), expresses in all the tissues tested, with the highest level in intestine, and responds to bacterial challenge in acute phase. We also expressed zITLN1 in E. coli system, and purified recombinant zITLN1 could agglutinate both Gram-positive and Gram-negative bacteria in a calcium dependent manner. Its ability to agglutinate Gram-positive bacteria is stronger than that to Gram-negative bacteria whereas zITLN2 did not show such preference. This is probably due to the fact that recombinant zITLN1 could bind peptidoglycan (PGN) with a higher degree to lipopolysaccharide (LPS). Our results of zITLN1 provided new insight into the evolution and function of the intelectin family.