MODULATION OF SYSTEMIC AND MUCOSAL IMMUNE-RESPONSES TO INHALED RAGWEED ANTIGEN IN EXPERIMENTALLY INDUCED INFECTION WITH RESPIRATORY SYNCYTIAL VIRUS IMPLICATION IN VIRALLY INDUCED ALLERGY
MODULATION OF SYSTEMIC AND MUCOSAL IMMUNE-RESPONSES TO INHALED RAGWEED ANTIGEN IN EXPERIMENTALLY INDUCED INFECTION WITH RESPIRATORY SYNCYTIAL VIRUS IMPLICATION IN VIRALLY INDUCED ALLERGY
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DOI：
10.1159/000234615
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发表时间：
1988-05-01
期刊：
影响因子：
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通讯作者：
OGRA, PL
中科院分区：
文献类型：
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作者：
LEIBOVITZ, E;FREIHORST, J;OGRA, PL
BALB/c小鼠被假感染或鼻内感染呼吸道合胞病毒（RSV）。在鼻内接种后第3天，各组小鼠连续吸入豚草抗原5 d，第31天再给豚草。采用ELISA法检测豚草抗原抗体活性的发展，并定期在血清和支气管洗涤中检测IgG和IgA抗体活性，以及被动皮肤过敏反应检测ige特异性反应。与假感染对照组相比，先前感染RSV的小鼠在初次接触豚草后血清IgG和IgE抗体反应水平显著提高。此外，在rsv感染的动物中，豚草的血清IgE反应较早上升。与对照组相比，rsv感染动物的支气管洗涤液中IgG和IgA抗豚草抗体活性也显著提高，尽管两组动物的支气管洗涤液中抗原特异性IgE活性也有类似的增加。这些发现支持这样一种可能性，即呼吸道粘膜限制性病毒感染可能会增强致敏性的发展，以及对急性感染期间呼吸道中同时发现的其他吸入过敏原的抗体反应的强度。
Groups of BALB/c mice were either sham-infected or infected intranasally with respiratory syncytial virus (RSV). On the third day following intranasal inoculation, all groups of mice were exposed by inhalation to ragweed antigen for 5 consecutive days and rechallenged with ragweed on day 31. Development of antibody activity to ragweed antigen was examined in serum and bronchial washings at regular intervals employing an ELISA assay for IgG and IgA antibody activity and passive cutaneous anaphylaxis for IgE-specific responses. The serum IgG and IgE antibody response to ragweed following primary exposure developed at significantly higher levels in mice previously infected with RSV, compared to sham-infected controls. In addition, an earlier rise in serum IgE response to ragweed occurred in the RSV-infected animals. IgG and IgA anti-ragweed antibody activity in bronchial washings was also observed with significantly higher levels in the RSV-infected animals when compared to the controls, although a similar increase in antigen-specific IgE activity in bronchial washings was found in both groups of animals. These findings support the possibility that mucosally restricted virus infections of the respiratory tract may enhance the development of sensitization and the magnitude of antibody responses to other inhaled allergens found concomitantly in the respiratory tract during acute infection.