Natural fluctuation and gonadal hormone regulation of astrocyte immunoreactivity in dentate gyrus.
Natural fluctuation and gonadal hormone regulation of astrocyte immunoreactivity in dentate gyrus.
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DOI：
10.1002/neu.480240705
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发表时间：
1993-07
期刊：
影响因子：
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通讯作者：
Sonia Luquín;Frederick Naftolin;Luis M. Garcia-Segura
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文献类型：
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作者：
Sonia Luquín;Frederick Naftolin;Luis M. Garcia-Segura
在不同的发情周期、卵巢切除后、雌二醇和/或黄体酮给药后，研究了成年雌性大鼠齿状回门和小脑皮层蚓部颗粒层对星形胶质细胞特异性标记物胶质纤维酸性蛋白（GFAP）免疫反应的细胞数量和表面密度。虽然不同实验组间免疫反应细胞数量无显著差异，但其表面密度在齿状回门区有显著变化。免疫反应细胞表面密度在发情期下午和发情期上午较发情期、发情期和去秋期上午升高，卵巢切除后降低，且在去卵巢大鼠单独注射17 -雌二醇（1、10、300微克/只大鼠）或与黄体酮（500微克/只大鼠）时呈剂量依赖性升高。相比之下，它不受17 α雌二醇（300微克/只大鼠）的影响。17 -雌二醇（300微克/大鼠）注射后5小时和黄体酮注射后1小时，免疫反应细胞表面密度较对照组显著增加。单独注射雌二醇或黄体酮对免疫反应细胞表面密度的影响比两种激素同时注射的影响要小。gfap -免疫反应细胞表面密度在17 -雌二醇和/或孕酮给药后24 h达到最大值，在孕酮和17 -雌二醇联合注射后48 h恢复到对照水平，而在仅注射两种激素中的一种的大鼠中，免疫反应细胞表面密度保持在对照值以上至少9天。在小脑皮层中没有观察到激素对gfap免疫反应细胞的影响。
The number and the surface density of cells immunoreactive for the specific astrocytic marker glial fibrillary acidic protein (GFAP), were evaluated in both the hilus of the dentate gyrus and the granular layer of the vermis of the cerebellar cortex of adult female rats during the different phases of the estrous cycle, after ovariectomy and after the pharmacological administration of estradiol and/or progesterone to ovariectomized rats. Although no significant differences were detected in the number of immunoreactive cells among the different experimental groups studied, their surface density showed significant changes in the hilus of the dentate gyrus. The surface density of immunoreactive cells was increased in the afternoon of proestrus and on the morning of estrus compared to the morning of proestrus, diestrus, and metestrus, was decreased after ovariectomy, and showed a dose-dependent increase in ovariectomized rats injected with 17 beta estradiol (1, 10, or 300 micrograms/rat), alone or in combination with progesterone (500 micrograms/rat). In contrast, it was not affected by the administration of 17 alpha estradiol (300 micrograms/rat). The surface density of immunoreactive cells was significantly increased over control values by 5 h after the injection of 17 beta estradiol (300 micrograms/rat) and as early as 1 h after the administration of progesterone. The separate injection of either 17 beta estradiol or progesterone had smaller effects on the surface density of immunoreactive cells than did the administration of both hormones together. The surface density of GFAP-immunoreactive cells reached maximal values by 24 h after the administration of 17 beta estradiol and/or progesterone and returned to control levels by 48 h after the combined injection of progesterone and 17 beta estradiol, while in the rats that were injected with only one of the two hormones, the surface density of immunoreactive cells remained over control values for at least 9 days. No such hormonal effects on GFAP-immunoreactive cells were observed in the cerebellar cortex.