IL-12-encoding plasmid has a beneficial, effect on spontaneous autoimmune disease in MRL/MP-lpr/lpr mice
IL-12-encoding plasmid has a beneficial, effect on spontaneous autoimmune disease in MRL/MP-lpr/lpr mice
复制标题
DOI：
10.1006/cyto.1999.0662
复制
发表时间：
2000-07-01
期刊：
影响因子：
3.8
通讯作者：
Ishigatsubo, Y
中科院分区：
文献类型：
--
作者：
Hagiwara, E;Okubo, T;Ishigatsubo, Y
Systemic lupus erythematosus (SLE) is characterized by immune abnormalities explained by the overproduction of Th-2 cytokines such as autoantibody production and polyclonal B cell activation. We examined the effect of administering a DNA plasmid encoding IL-12 on the lupus-like disease of MRL/MP-lpr/lpr (MRL/lpr) mice. Treatments were delivered intramuscularly every 4 weeks, starting at 4 weeks of age. This intervention significantly inhibited the accumulation of CD4(-) CD8(-) T cells, and reduced lymphadenopathy and splenomegaly, A significant decrease in serum IgG anti-DNA autoantibody titers was observed, and plasmid IL-12 therapy was also associated with a reduction in the proteinuria and glomerulonephritis characteristic of this disease, Serum IFN-gamma level was increased by inoculating IL-12 encoding plasmid, suggesting that the cytokine balance was skewed towards Th-1, The clinical implications of this suppression of autoimmune disease are also discussed. (C) 2000 Academic Press.
