AcMNPV-miR-3 is a miRNA encoded by Autographa californica nucleopolyhedrovirus and regulates the viral infection by targeting ac101
AcMNPV-miR-3 is a miRNA encoded by Autographa californica nucleopolyhedrovirus and regulates the viral infection by targeting ac101
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DOI：
10.1016/j.virusres.2019.05.004
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发表时间：
2019-07-02
期刊：
影响因子：
5
通讯作者：
Wang, Xunzhang
中科院分区：
文献类型：
--
作者：
Jiao, Yingzhen;Wang, Jinwen;Wang, Xunzhang
MicroRNAs （miRNAs）是一种存在于植物、动物和许多病毒中的小的非编码rna，它调节着各种生物过程。本小组此前报道了加州自签名多核多角体病毒（AcMNPV）编码的第一个miRNA， AcMNPV- mir -1，它调节三种病毒基因的表达。本研究表征了由AcMNPV编码的另一种miRNA， AcMNPV- mir -3。该miRNA位于AcMNPV基因组中病毒基因ac101编码序列的相反链上，在感染后6小时可以检测到，在感染后12小时左右在AcMNPV感染的Sf9细胞中积累到峰值。5个病毒基因（ac101、ac23、ac25、ac86和ac98）被证实受AcMNPV-miR-3调控。AcMNPV-miR-3可能通过sirna样切割模式显著下调Ac101。过量的AcMNPV-miR-3导致感染性芽化病毒粒子（BV）的产生减少，并加速闭塞衍生病毒粒子（ODV）的形成。这些结果表明AcMNPV-miR-3可能在BV和ODV的产生中发挥调节作用。
MicroRNAs (miRNAs), which are small noncoding RNAs found in plants, animals, and many viruses, regulate various biological processes. Our group has previously reported the first miRNA encoded by Autographa californica multiple Nucleopolyhedrovirus (AcMNPV), AcMNPV-miR-1, which regulates the expression of three viral genes. This study characterizes another miRNA encoded by AcMNPV, AcMNPV-miR-3. This miRNA is located on the opposite strand of the viral gene ac101 coding sequence in the AcMNPV genome, and it can be detected at 6 h post-infection and accumulated to a peak around 12 h post-infection in AcMNPV infected Sf9 cells. Five viral genes (ac101, ac23, ac25, ac86, and ac98) were verified to be regulated by AcMNPV-miR-3. Ac101 was markedly down-regulated by AcMNPV-miR-3 that may be via a siRNA-like cleavage mode. Administrating excessive AcMNPV-miR-3 resulted in decreased production of infectious budded virions (BV) and accelerated the formation of occlusion-derived virions (ODV). These results suggest that AcMNPV-miR-3 may play a regulatory role in BV and ODV production.