Investigation of colorimetric biosensor array based on programable surface chemistry of M13 bacteriophage towards artificial nose for volatile organic compound detection: From basic properties of the biosensor to practical application
Investigation of colorimetric biosensor array based on programable surface chemistry of M13 bacteriophage towards artificial nose for volatile organic compound detection: From basic properties of the biosensor to practical application
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DOI：
10.1016/j.bios.2021.113339
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发表时间：
2021-05-21
影响因子：
12.6
通讯作者：
Oh, Jin-Woo
中科院分区：
文献类型：
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作者：
Lee, Jong-Min;Lee, Yujin;Oh, Jin-Woo
爆炸物、毒品、环境激素和变质食品等各种威胁都表现为我们环境中存在挥发性有机化合物（VOC）。为了尽早识别并应对这些威胁，对高灵敏度和选择性电子鼻的需求正在增加。基于M13噬菌体的光电鼻是满足所有这些要求的绝佳候选者。然而，基于噬菌体的电子鼻仍处于起步阶段，包括系统方法和开发在内的策略仍然至关重要。在这里，我们已经集成了理论和实验方法来分析基因工程噬菌体的表面化学和基于噬菌体的光电鼻属性之间的相关性。定量分析了基因工程噬菌体彩色膜对某些挥发性有机化合物的反应性，并与密度泛函理论（DFT）计算的结合亲和力值进行了相关性比较。这表明噬菌体彩色膜通过基因工程具有可控的反应性。我们已经选择了在这项工作中，通过层次聚类分析（HCA），有利于区分每一种VOCs。通过DFT计算的优化几何构型验证了这种差异的原因。通过这一点，它被证实，基于组氨酸和基于基因工程噬菌体膜是重要的，在区分挥发性有机化合物（γ-己内酯，2-异丙基-4-甲基噻唑，乙醇，丙酮，乙酸乙酯，乙醛）在这项工作中使用，以评估桃新鲜度品质。这被应用到一个现场应用的噬菌体为基础的光电鼻的设计，并通过测量实际水果的新鲜度进行验证。
Various threats such as explosives, drugs, environmental hormones, and spoiled food manifest themselves with the presence of volatile organic compounds (VOCs) in our environment. In order to recognize and respond to these threats early, the demand for highly sensitive and selective electronic noses is increasing. The M13 bacteriophage-based optoelectronic nose is an excellent candidate to meet all these requirements. However, the phage-based electronic nose is still in its infancy, and strategies that include a systematic approach and development are still essential. Here, we have integrated theoretical and experimental approaches to analyze the correlation between the surface chemistry of genetically engineered phage and the phage-based optoelectronic nose properties. The reactivity of the genetically engineered phage color film to some VOCs were quantitatively analyzed, and the correlation with the binding affinity value calculated by Density-functional theory (DFT) was compared. This demonstrates that phage color films have controllable reactivity through a genetic engineering. We have selected phages that are advantageous in distinguishing each VOCs in this work through hierarchical cluster analysis (HCA). The reason for this difference was verified through the optimized geometry calculated by DFT. Through this, it was confirmed that the tryptophan-based and the Histidine-based of genetically engineered phage film are important in distinguishing the VOCs (Y-hexanolactone, 2-isopropyl-4-methylthiazole, ethanol, acetone, ethyl acetate, and acetaldehyde) used in this work to evaluate the peach freshness quality. This was applied to the design of a field-applied phage-based optoelectronic nose and verified by measuring the freshness of the actual fruit.