Mechanism of decreased in vitro murine macrophage cytokine release after exposure to carbon dioxide - Relevance to laparoscopic surgery
Mechanism of decreased in vitro murine macrophage cytokine release after exposure to carbon dioxide - Relevance to laparoscopic surgery
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DOI：
10.1097/00000658-199708000-00010
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发表时间：
1997-08-01
影响因子：
9
通讯作者：
Rotstein, OD
中科院分区：
文献类型：
--
作者：
West, MA;Hackam, DJ;Rotstein, OD
目的研究二氧化碳（CO2）对腹腔巨噬细胞功能的影响。背景资料腹腔镜手术与轻微疼痛、发热和低水平炎性细胞因子相关。为了了解其中的机制，作者研究了不同气体对小鼠腹膜巨噬细胞胞内pH的影响，并将这些变化与lps刺激的炎症细胞因子释放的变化联系起来。方法腹腔巨噬细胞在空气、氦气、CO2中孵育2 h，比较实验气体对当日或次日脂多糖刺激的肿瘤坏死因子（TNF）和白细胞介素- 1释放的影响。利用单细胞荧光成像技术测定巨噬细胞暴露于试验气体中的胞浆pH值。在麻醉大鼠腹腔灌气过程中测定了实验气体的体内效应。结果CO2培养的巨噬细胞产生的TNF和白细胞介素-1明显低于空气或氦气培养的巨噬细胞。细胞因子的产生在24小时后恢复正常。暴露在二氧化碳中，而不是空气或氦气中，会引起明显的细胞质酸化。细胞内酸化的药理学诱导达到相似的水平，再现了抑制作用。体外研究表明，CO2注入降低了组织pH值和腹腔巨噬细胞lps刺激的TNF生成。结论腹腔镜手术时，腹膜CO2注入引起的细胞酸化有助于减弱局部炎症反应。
ObjectiveThe objective of this study was to determine the effect of carbon dioxide (CO2) on the function of peritoneal macrophages.Summary Background DataLaparoscopic surgery is associated with minimal pain, fever, and low levels of inflammatory cytokines. To understand the mechanisms involved, the authors investigated the effect of different gases on murine peritoneal macrophage intracellular pH and correlated these alterations with alterations in LPS-stimulated inflammatory cytokine release.MethodsPeritoneal macrophages were incubated for 2 hours in air, helium, or CO2, and the effect of the test gas on immediate or next day lipopolysaccharide (LPS)-stimulated tumor necrosis factor (TNF) and interleukin-l release compared. Cytosolic pH of macrophages exposed to test gases was measured using single-cell fluorescent imaging. The in vivo effects of test gases were determined in anesthetized rats during abdominal insufflation.ResultsMacrophages incubated in CO2 produced significantly less TNF and interleukin-1 in response to LPS compared to incubation in air or helium. Cytokine production returned to normal 24 hours later. Exposure to CO2, but not air or helium, caused a marked cytosolic acidification. Pharmacologic induction of intracellular acidification to similar levels reproduced the inhibitory effect. In vitro studies showed that CO2 insufflation lowered tissue pH and peritoneal macrophage LPS-stimulated TNF production.ConclusionsThe authors propose that cellular acidification induced by peritoneal CO2 insufflation contributes to blunting of the local inflammatory response during laparoscopic surgery.