Determination of paclitaxel in human plasma following the administration of Genaxol or Genetaxyl by liquid chromatography/tandem mass spectrometry
Determination of paclitaxel in human plasma following the administration of Genaxol or Genetaxyl by liquid chromatography/tandem mass spectrometry
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DOI：
10.1002/rcm.2577
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发表时间：
2006-01-01
影响因子：
2
通讯作者：
Sparreboom, Alex
中科院分区：
文献类型：
--
作者：
Gardner, Erin R.;Liau, Chi-Ting;Sparreboom, Alex
建立了一种灵敏、特异的紫杉醇血药浓度测定方法，以克服先前发表的分析方法的局限性，采用高效液相色谱-串联质谱仪检测紫杉醇。以[H-2(5)]紫杉醇为内标，采用1-氯丁烷/乙腈(4：1，v/v)液-液萃取法测定血浆中紫杉醇的含量。色谱柱为Waters SymmetryShield C8柱(50×2.1 mm，3.5微米)。总运行时间，包括平衡，使用乙腈和10 mM甲酸铵的梯度，pH 4.0，为8分钟。该方法在2-2500 ng/m L范围内具有较高的准确度和精密度，已成功应用于紫杉醇口服和静脉给药的临床药代动力学研究。版权所有(C)2006 John Wiley&Sons，Ltd.
A sensitive and specific assay for paclitaxel in plasma has been developed to overcome limitations in previously published assays, using liquid chromatography with tandem mass spectrometric detection. Plasma samples (100 mu L) were subjected to liquid-liquid extraction with 1-chlorobutane/acetonitrile (4:1, v/v), with [H-2(5)]paclitaxel employed as the internal standard. Chromatography was carried out with a Waters SymmetryShield C8 column (50 X 2.1 mm, 3.5 mu m). The total run time, including equilibration, was 8 min, using a gradient of acetonitrile and 10 mM ammonium formate, pH 4.0. The assay is accurate and precise over the range of 2-2500 ng/mL and has been successfully applied to study the clinical pharmacokinetics of two formulations of paclitaxel, Genaxol and Genetaxyl, given orally and intravenously. Copyright (c) 2006 John Wiley & Sons, Ltd.