A novel approach to blood plasma viscosity measurement using fluorescent molecular rotors
A novel approach to blood plasma viscosity measurement using fluorescent molecular rotors
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DOI：
10.1152/ajpheart.00712.2001
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发表时间：
2002-05-01
影响因子：
4.8
通讯作者：
Intaglietta, M
中科院分区：
文献类型：
--
作者：
Haidekker, MA;Tsai, AG;Intaglietta, M
分子转子是一组具有粘度依赖性量子产率的荧光分子，测试了它们作为基于荧光的等离子体粘度计的适用性。通过加入pentastarch（分子量260 kDa，10%盐水溶液）改变人血浆样品的粘度，并用Brookfield粘度计测量。血浆粘度为1.6 mPa.s，混合物范围高达4.5 mPa.s（21 ℃）。混合在血浆样品中的分子转子的受激光发射产生的光强度是不重叠的，并且对于低至0.3mPa.s的粘度阶跃具有显著不同的强度（n = 5，P < 0.0001）。强度（I）与粘度（η）之间的数学关系为η =（κI）（ν）。在校准和缩放之后，基于荧光的测量相对于常规粘度测量具有平均偏差，
Molecular rotors, a group of fluorescent molecules with viscosity-dependent quantum yield, were tested for their suitability to act as fluorescence-based plasma viscometers. The viscosity of samples of human plasma was modified by the addition of pentastarch (molecular mass 260 kDa, 10% solution in saline) and measured with a Brookfield viscometer. Plasma viscosity was 1.6 mPa.s, and the mixtures ranged up to 4.5 mPa.s (21degreesC). The stimulated light emission of the molecular rotors mixed in the plasma samples yielded light intensity that was nonoverlapping and of significantly different intensity for viscosity steps down to 0.3 mPa.s (n = 5, P < 0.0001). The mathematical relationship between intensity (I) and viscosity (η) was found to be η = (κI)(ν). After calibration and scaling the fluorescence based measurement had an average deviation versus the conventional viscometric measurements that was