Molecular and cellular characterization of ABCG2 in the prostate.
Molecular and cellular characterization of ABCG2 in the prostate.
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ABCG2在前列腺中的分子和细胞表征。
DOI：
10.1186/1471-2490-7-6
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发表时间：
2007-04-10
期刊：
影响因子：
2
通讯作者：
Liu AY
中科院分区：
文献类型：
--
作者：
Pascal LE;Oudes AJ;Petersen TW;Goo YA;Walashek LS;True LD;Liu AY
前列腺干细胞的鉴定和表征对于了解正常前列腺发育和癌变是重要的。基于流式细胞术的侧群（SP）技术已被开发用于分离包括前列腺在内的几种人体组织类型中的假定成体干细胞。这种表型主要由ATP结合盒膜转运蛋白ABCG 2介导。在从根治性前列腺切除术获得的正常前列腺组织上进行ABCG 2的免疫定位。分离正常人前列腺SP细胞和ABCG 2+细胞，用DNA芯片分析和RT-PCR检测基因表达。通过用CD 31预分选去除内皮细胞。ABCG 2阳性细胞定位于前列腺基底上皮和内皮。基底上皮中的ABCG 2+细胞占总基底细胞群的不到1%。SP细胞占上皮细胞总数的0.5-3%。SP转录组与ABCG 2+基本相同，两个群体均表达指示干细胞表型的基因，然而，细胞也表达许多与内皮细胞共同的基因。这些结果提供了前列腺SP和ABCG 2+细胞的基因表达谱，这对于研究正常发育和致癌作用，特别是与癌症干细胞概念相关的研究至关重要。
Identification and characterization of the prostate stem cell is important for understanding normal prostate development and carcinogenesis. The flow cytometry-based side population (SP) technique has been developed to isolate putative adult stem cells in several human tissue types including the prostate. This phenotype is mainly mediated by the ATP-binding cassette membrane transporter ABCG2. Immunolocalization of ABCG2 was performed on normal prostate tissue obtained from radical prostatectomies. Normal human prostate SP cells and ABCG2+ cells were isolated and gene expression was determined with DNA array analysis and RT-PCR. Endothelial cells were removed by pre-sorting with CD31. ABCG2 positive cells were localized to the prostate basal epithelium and endothelium. ABCG2+ cells in the basal epithelium constituted less than 1% of the total basal cell population. SP cells constituted 0.5–3% of the total epithelial fraction. The SP transcriptome was essentially the same as ABCG2+ and both populations expressed genes indicative of a stem cell phenotype, however, the cells also expressed many genes in common with endothelial cells. These results provide gene expression profiles for the prostate SP and ABCG2+ cells that will be critical for studying normal development and carcinogenesis, in particular as related to the cancer stem cell concept.