Roles of hypothalamic subgroup histamine and orexin neurons on behavioral responses to sleep deprivation induced by the treadmill method in adolescent rats.
Roles of hypothalamic subgroup histamine and orexin neurons on behavioral responses to sleep deprivation induced by the treadmill method in adolescent rats.
复制标题
DOI：
10.1254/jphs.10177fp
复制
发表时间：
2010
影响因子：
3.5
通讯作者：
A. Xu;E. Sakurai;Atsuo Kuramasu;Jian Zhang;Jiyu Li;N. Okamura;Dongying Zhang;T. Yoshikawa;Takehiko Watanabe;K. Yanai
中科院分区：
文献类型：
--
作者：
A. Xu;E. Sakurai;Atsuo Kuramasu;Jian Zhang;Jiyu Li;N. Okamura;Dongying Zhang;T. Yoshikawa;Takehiko Watanabe;K. Yanai
睡眠剥夺会对行为、情绪、注意力和学习能力产生一些负面影响。睡眠在青少年大脑发育过程中显得尤为重要。在本研究中，我们研究了睡眠剥夺对青春期大鼠的行为和下丘脑神经传递，包括组胺和食欲素神经元的影响，采用跑台法。将青春期雄性大鼠分为三组：跑步机睡眠剥夺组、跑步机对照组和笼内对照组。能量消耗，焦虑样行为，和自发活动之间的三组进行了检查。还检测了皮质和间脑中的组胺浓度以及下丘脑中c-Fos阳性神经元的数量。此外，组胺和食欲素神经元在下丘脑同时确定分别使用大鼠组氨酸脱羧酶和食欲素-A免疫组化。实验性3天睡眠剥夺显著增加了能量消耗和焦虑相关行为，而探索性运动活动显著减少。皮层组胺含量无明显变化，间脑组胺含量显著降低。在下丘脑中观察到c-Fos阳性神经元的表达增加，包括组胺和食欲素神经元亚群。这些发现表明，睡眠剥夺增加了青春期大鼠的能量消耗和焦虑，并为下丘脑亚群组胺和食欲素神经元在睡眠剥夺行为反应中的关键作用提供了证据。
Sleep deprivation induces several negative effects on behavior, emotion, attention, and learning ability. Sleep appears to be particularly important during adolescent brain development. In the present study, we examined the effects of sleep deprivation on behavior and hypothalamic neurotransmission including histamine and orexin neurons in adolescent rats using the treadmill method. Adolescent male rats were divided into three groups: treadmill sleep-deprived, treadmill control, and cage control groups. Energy expenditure, anxiety-like behavior, and locomotor activity were examined among the three groups. Histamine concentration in the cortex and diencephalon and the number of c-Fos-positive neurons in the hypothalamus were also examined. In addition, histamine and orexin neurons in the hypothalamus were simultaneously identified using rat histidine decarboxylase and orexin-A immunohistochemistry, respectively. Both energy expenditure and anxiety-related behavior significantly increased by the experimental 3-day sleep deprivation, while exploratory locomotor activity significantly decreased. Histamine contents did not change in the cortex, but significantly decreased in the diencephalon of sleep-deprived rats. Increased expression of c-Fos-positive neurons, including subgroup histamine and orexin neurons, was observed in the hypothalamus. These findings indicate that sleep deprivation increases energy expenditure and anxiety in adolescent rats and provide evidence for the pivotal role of hypothalamus subgroup histamine and orexin neurons in the behavioral response to sleep deprivation.