Donnan membrane equilibrium, sedimentation equilibrium, and coil expansion of DNA in salt solutions.
Donnan membrane equilibrium, sedimentation equilibrium, and coil expansion of DNA in salt solutions.
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盐溶液中 DNA 的唐南膜平衡、沉降平衡和线圈扩张。
DOI：
10.1007/bf02797120
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Stigter,D
中科院分区：
文献类型：
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作者：
Stigter,D
本文综述了McMillan-Mayer-Hill维里理论和离子双电层理论在稀胶体溶液，特别是DNA溶液中的应用。高电荷胶体棒之间的相互作用的第二维里系数的两个杆，一个杆和一个小的co-ion。基于Poisson-Boltzmann方程，用相互作用势计算了相关的团簇积分。处理扩展到负责卷曲DNA的统计学膨胀（排除体积效应）的链内排斥。该理论与三组实验数据进行了比较：在Donnan膜平衡的DNA-盐溶液中的盐分布，在不同的离子强度下的短DNA片段的沉降平衡，和T7 DNA在NaCl溶液中的特性粘度。在所有情况下，理论与实验都很符合.该协议是没有说服力的沉降平衡在低离子强度，因为在这里的实验DNA浓度太高，截断稀溶液膨胀的DNA-盐排斥。
This is a review of applications of the McMillan-Mayer-Hill virial theory and the ionic double-layer theory to dilute colloidal solutions, in particular, solutions of DNA. Interactions of highly charged colloidal rods are developed in terms of the second virial coefficients between two rods, and between one rod and one small co-ion. The relevant cluster integrals are evaluated with interaction potentials based on the Poisson-Boltzmann equation. The treatment is extended to the intrachain repulsion responsible for the statistical swelling of coiled DNA (excluded volume effect). The theory is compared with three sets of experimental data: The salt distribution in Donnan membrane equilibria of DNA-salt solutions, sedimentation equilibria of short DNA fragments at different ionic strengths, and the intrinsic viscosity of T7 DNA in NaCl solutions. In all cases the theory agrees well with the experiments. The agreement is not convincing for the sedimentation equilibrium at low ionic strength, because here the experimental DNA concentration is too high for the truncated dilute solution expansion of the DNA-salt repulsion.