Expression of 3β-hydroxysteroid dehydrogenase (hsd3b), star and ad4bp/sf-1 during gonadal development in medaka (Oryzias latipes)
Expression of 3β-hydroxysteroid dehydrogenase (hsd3b), star and ad4bp/sf-1 during gonadal development in medaka (Oryzias latipes)
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DOI：
10.1016/j.ygcen.2012.01.019
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发表时间：
2012-04-01
影响因子：
2.7
通讯作者：
Shibata, Naoki
中科院分区：
文献类型：
--
作者：
Nakamoto, Masatoshi;Fukasawa, Motoaki;Shibata, Naoki
在大多数脊椎动物中，性类固醇在性腺发育、生殖细胞成熟和第二性征发育中起着关键作用。性类固醇在睾丸中的类固醇产生细胞（SPC）中合成，称为Leydig细胞，以及卵巢中的卵泡膜细胞和颗粒细胞。在PC中，胆固醇依次被一组类固醇生成因子和酶催化以产生性类固醇。因此，类固醇合成相关基因的整合表达对于性类固醇的正常生产至关重要。在本研究中，类固醇生成因子和酶的调节机制进行了检查。本研究重点研究了青鳉hsd 3b、星星和ad 4 bp/sf-1基因在性腺发育过程中的时空表达。在睾丸发育过程中，hsd 3b、星星和ad 4 bp/sf-1在生精小管前体形成后的间质体细胞中共表达，表明ad 4 bp/sf-1调节hsd 3b和星星的转录。在卵巢发育过程中，hsd 3b的表达模式与cyp 11 a1的表达模式一致，但与芳香化酶的表达模式不一致。虽然ad 4 bp/sf-1主要表达于推定的卵泡细胞，但在发育中的卵巢中hsd 3b阳性的间质细胞中也检测到ad 4 bp/sf-1。与我们的预期相反，星星的表达发生在卵巢发育的后期，而不是其他类固醇生成酶的表达。因此，星星转录的调节机制似乎与发育中的卵巢中的其他类固醇生成酶的调节机制不同，但在发育中的睾丸中没有。(C)2012 Elsevier Inc. All rights reserved.
In most vertebrates, sex steroids play a critical role in gonadal development, maturation of germ cells, and development of secondary sexual characteristics. Sex steroids are synthesized in steroid-producing cells (SPCs) in the testis known as Leydig cells, as well as in thecal and granulosa cells in the ovary. In PCs, cholesterol is sequentially catalyzed by a set of steroidogenic factors and enzymes in order to produce sex steroids. Therefore, integrated expression of the genes involved in steroidogenesis is critical for the proper production of sex steroids. In the present study, regulatory mechanisms of steroidogenic factors and enzymes were examined. We focused on hsd3b, star and ad4bp/sf-1 as well as the description of temporal and spatial expression of these genes during gonadal development in medaka (Oryzias latipes). During testicular development, hsd3b, star and ad4bp/sf-1 were co-expressed in the interstitial somatic cells subsequent to the formation of the seminiferous tubule precursor, suggesting that ad4bp/sf-1 regulated the transcription of both hsd3b and star. During ovarian development, the expression pattern of hsd3b coincided with that of cyp11a1, but not with that of aromatase. Although ad4bp/sf-1 was mainly expressed in presumptive follicular cells, it was also detected in hsd3b positive interstitial cells in the developing ovary. Contrary to our expectations, the onset of star expression occurred during a later stage of ovarian development than the expression of other steroidogenic enzymes. Thus, the regulation mechanism of star transcription appears to differ from that of the other steroidogenic enzymes in the developing ovary, but not in the developing testis. (C) 2012 Elsevier Inc. All rights reserved.