Expression of the p53 homologue p63alpha and deltaNp63alpha in normal and neoplastic cells.
Expression of the p53 homologue p63alpha and deltaNp63alpha in normal and neoplastic cells.
复制标题
p53 同源物 p63alpha 和 deltaNp63alpha 在正常和肿瘤细胞中的表达。
作者：
P. Hall;S. Campbell;M. O'Neill;D. Royston;K. Nylander;F. Carey;N. Kernohan
最近描述了一个新兴的p53相关基因家族，包括p73和p63。这两个基因编码的蛋白质与p53有许多相似之处，但也有可能通过替代启动子的使用和替代剪接形成一系列相关物种。为了开始对p63的鉴定，我们制备了一种识别p63αC末端的多克隆血清(命名为SC1)。我们已经证明该试剂识别p63α，但不识别p53或p73。经Western印迹分析，在一系列细胞系中均发现p63α和p63αN端截短形式(DeltaNp63α)。类似的组织免疫印迹分析显示相当复杂，至少有四种SC1免疫反应异构体被鉴定出来。在免疫组织学研究中，SC1免疫反应被广泛检测到，主要与上皮细胞中的增殖室有关。然而，非增殖性人群也可以显示SC1免疫染色。组织裂解物的免疫印迹鉴定的异构体与组织免疫染色特征之间没有简单的关系。在包括神经和外周血淋巴细胞在内的几个组织中发现了一种以前未被发现的介于p63α和DeltaNp63α之间的迁移率中间物种。有趣的是，紫外线和MMS处理后，HaCat细胞中p63α的表达受到时间和浓度依赖的抑制。我们的数据提供了关于p63复杂性的进一步信息，SC1血清将被证明是进一步研究该p53同源物的有用工具。
A burgeoning family of p53-related genes have been described recently, including p73 and p63. Both these genes encode proteins with many similarities to p53 but also with the potential for forming a range of related species by alternative promoter usage and alternative splicing. In order to begin the characterization of p63, we generated a polyclonal serum (designated SC1) that recognizes the C-terminus of p63alpha. We have shown that this reagent recognizes p63alpha but not p53 nor p73. By western blot analysis both p63alpha and the N-terminal truncated form of p63alpha (DeltaNp63alpha) were found in a range of cell lines. Similar immunoblot analysis of tissues reveals considerable complexity with at least four SC1-immunoreactive isoforms being identified. In immunohistological studies SC1 immunoreactivity is widely detectable, being predominantly associated with proliferative compartments in epithelia. However, non-proliferative populations can also show SC1 immunostaining. No simple relationship between the isoforms identified by immunoblotting of tissue lysates and the tissue immunostaining characteristics was identified. A previously unrecognized species intermediate in mobility between p63alpha and DeltaNp63alpha was found in several tissues, including nerve and peripheral blood lymphocytes. Interestingly, there is suppression of p63alpha expression in HaCat cells in a time- and concentration-dependent manner after UV and MMS treatment. Our data provide further information about the complexity of p63 and the SC1 serum will prove to be a useful tool in further studies of this p53 homologue.
影响因子：
11.2
作者：
Jian‐hui Zhu;Jieyuan Jiang;Wenjing Zhou;Xinbin Chen
通讯作者：
Jian‐hui Zhu;Jieyuan Jiang;Wenjing Zhou;Xinbin Chen